Typical Testing Data/Standard Curve (for reference only)
(Mouse Endostatin PicoKine ELISA Kit standard curve )
Collagen alpha-1(XVIII) chain ELISA Kit | COL18A1 elisa kit
Mouse Endostatin PicoKine ELISA Kit
Reactivity
Mouse. There is no detectable cross-reactivity with other relevant proteins.
Synonyms
Collagen alpha-1(XVIII) chain; Mouse Endostatin PicoKine ELISA Kit; Alpha 1 collagen type 18 (XVIII)(COL18A1); Alpha 1 type XVIII collagen; Antiangiogenic agent; COIA1_HUMAN; COL15A1; COL18A1; Collagen alpha 1(XV) chain; Collagen alpha 1(XVIII) chain; Collagen alpha-1(XV) chain; Collagen type XV proteoglycan; Collagen type XVIII alpha 1; Collagen XV; alpha 1 polypeptide; Collagen; type XV; alpha 1; Endostatin; Endostatin XV; FLJ27325; FLJ34914; FLJ38566; KNO; KNO1; KS; MGC74745; Multi functional protein MFP; OTTHUMP00000021782; OTTHUMP00000115472; OTTHUMP00000115473; collagen; type XVIII; COL18A1 elisa kit
Reactivity
Mouse. There is no detectable cross-reactivity with other relevant proteins.
Sequence Length
1527
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Sensitivity
<10pg/ml
Immunogen
NSO, H1591-K1774
Intra-Assay Precision
Sample 1: n=16; Mean(ng/ml): 0.8; Standard deviation: 0.052; CV(%): 6.5 Sample 2: n=16; Mean(ng/ml): 2.14; Standard deviation: 0.135; CV(%): 6.3 Sample 3: n=16; Mean(ng/ml): 4.25; Standard deviation: 0.179; CV(%): 4.2
Inter-Assay Precision
Sample 1: n=24; Mean(ng/ml): 0.84; Standard deviation: 0.06; CV(%): 7.1 Sample 2: n=24; Mean(ng/ml): 2.23; Standard deviation: 0.147; CV(%): 6.6 Sample 3: n=24; Mean(ng/ml): 4.37; Standard deviation: 0.253; CV(%): 5.8
Assay Range
156pg/ml-10,000pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse Endostatin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse Endostatin PicoKine ELISA Kit standard curve )
Related Product Information for COL18A1 elisa kit
Description: Sandwich ELISA kit of Quantitative Detection for Mouse Endostatin
Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134,204 Da
NCBI Official Full Name
collagen alpha-1(XVIII) chain isoform 1
NCBI Official Synonym Full Names
collagen, type XVIII, alpha 1
NCBI Official Symbol
Col18a1
NCBI Protein Information
collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; procollagen, type XVIII, alpha 1
UniProt Protein Name
Collagen alpha-1(XVIII) chain
Protein Family
UniProt Gene Name
Col18a1
UniProt Entry Name
COIA1_MOUSE
Uniprot Description
COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; basement membrane
Molecular Function: identical protein binding; metal ion binding; structural molecule activity
Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; positive regulation of cell migration
Research Articles on COL18A1
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Product Notes
The Mouse. There is no detectable cross-reactivity with other relevant proteins. COL18A1 col18a1 (Catalog #AAA177551) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177551 ELISA Kit recognizes Mouse. There is no detectable cross-reactivity with other relevant proteins. COL18A1. It is sometimes possible for the material contained within the vial of "Collagen alpha-1(XVIII) chain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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