SDS-Page
(Recombinant TRIM28 (624-811) protein gel. TRIM28 (624-811) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)
TRIM28 recombinant protein
Recombinant TRIM28 (624-811) protein
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Purity
The recombinant protein is >87% pure by SDS-PAGE.
Synonyms
TRIM28; Recombinant TRIM28 (624-811) protein; KAP1; TF1B; RNF96; TIF1B; PPP1R157; TRIM28 recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >87% pure by SDS-PAGE.
Form/Format
Recombinant TRIM28 (624-811) protein expressed in E Coli and supplied in 25mM Tris pH7.4, 150mM NaCl, 5% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant TRIM28 (624-811) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant TRIM28 (624-811) protein gel. TRIM28 (624-811) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant TRIM28 (624-811) protein gel. TRIM28 (624-811) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(Recombinant TRIM28 (624-811) activity assay. 3.3 uM histone peptide H4K5/8/12/16 (4ac) was incubated with TRIM28 (624-811) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(Recombinant TRIM28 (624-811) activity assay. 3.3 uM histone peptide H4K5/8/12/16 (4ac) was incubated with TRIM28 (624-811) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Related Product Information for TRIM28 recombinant protein
Short Description: The peptide corresponding to amino acids 624-811 that contains the bromodomain sequences of TRIM28 (accession number NP_005753.1) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 26.18 kDa. Tripartite motif-containing 28 (TRIM28) protein, also known as transcriptional intermediary factor 1b (TIF1b) and KAP1 (Krüppel-associated protein), is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes enhances transcriptional repression by coordinating the increase in TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM28 has been shown to function as a nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). It also mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at lysine 9) to the promoter regions of KRAB target genes. TRIM28 enhances transcriptional repression by coordinating the increase in H3K9 methylation, the decrease in H3K9 and H3K14 acetylation, respectively, and the disposition of HP1 proteins to silence gene expression. It also acts as a corepressor for ErbB-4. TRIM28 inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation and may serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of pRb. TRIM28 has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. TRIM28 also specifically SUMOylates IRF7, inhibiting its transactivation activity, and ubiquitinates p53 leading to its proteosomal degradation. Recombinant TRIM28 (624-811) can be used in binding assays and inhibitor screening.
Background: Tripartite motif-containing 28 (TRIM28) protein, also known as transcriptional intermediary factor 1b (TIF1b) and KAP1 (Krüppel-associated protein), is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes enhances transcriptional repression by coordinating the increase in TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM28 has been shown to function as a nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). It also mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at lysine 9) to the promoter regions of KRAB target genes. TRIM28 enhances transcriptional repression by coordinating the increase in H3K9 methylation, the decrease in H3K9 and H3K14 acetylation, respectively, and the disposition of HP1 proteins to silence gene expression. It also acts as a corepressor for ErbB-4. TRIM28 inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation and may serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of pRb. TRIM28 has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. TRIM28 also specifically SUMOylates IRF7, inhibiting its transactivation activity, and ubiquitinates p53 leading to its proteosomal degradation.
Background: Tripartite motif-containing 28 (TRIM28) protein, also known as transcriptional intermediary factor 1b (TIF1b) and KAP1 (Krüppel-associated protein), is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes enhances transcriptional repression by coordinating the increase in TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM28 has been shown to function as a nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). It also mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at lysine 9) to the promoter regions of KRAB target genes. TRIM28 enhances transcriptional repression by coordinating the increase in H3K9 methylation, the decrease in H3K9 and H3K14 acetylation, respectively, and the disposition of HP1 proteins to silence gene expression. It also acts as a corepressor for ErbB-4. TRIM28 inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation and may serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of pRb. TRIM28 has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. TRIM28 also specifically SUMOylates IRF7, inhibiting its transactivation activity, and ubiquitinates p53 leading to its proteosomal degradation.
Product Categories/Family for TRIM28 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,474 Da
NCBI Official Full Name
Homo sapiens tripartite motif containing 28 (TRIM28), mRNA
NCBI Official Synonym Full Names
tripartite motif containing 28
NCBI Official Symbol
TRIM28
NCBI Official Synonym Symbols
KAP1; TF1B; RNF96; TIF1B; PPP1R157
NCBI Protein Information
transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96; TIF1-beta; nuclear corepresso
Protein Family
NCBI Description
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
Research Articles on TRIM28
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Product Notes
The TRIM28 (Catalog #AAA388155) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TRIM28, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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