SDS-Page
(Recombinant KMT2B (MLL4)-SET protein gel. KMT2B (MLL4)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
KMT2B recombinant protein
Recombinant KMT2B (MLL4)-SET protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KMT2B (MLL4)-SET protein gel. KMT2B (MLL4)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant KMT2B (MLL4)-SET protein gel. KMT2B (MLL4)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant KMT2B (MLL4)-SET protein gel. KMT2B (MLL4)-SET was incubated 4 equimolar components of the MLL4 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours at 4 degree C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant KMT2B (MLL4)-SET protein gel. KMT2B (MLL4)-SET was incubated 4 equimolar components of the MLL4 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours at 4 degree C. All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.)
Background: KMT2B (MLL4, Myeloid/lymphoid or mixed-lineage leukemia)-SET is a Trithorax-group protein that function collectively to promote gene expression. KMT2B (MLL4)-SET is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. KMT2B (MLL4)-SET plays a central role in b-globin locus transcription regulation by being recruited by Nfe2. KMT2B (MLL4)-SET is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. KMT2B (MLL4)-SET may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known. [provided by RefSeq, Jul 2008]
Uniprot Description
MLL2: may be a transcriptional regulator. Contains 3 A.T hook DNA-binding domains. Two alternatively spliced isoforms have been described.
Protein type: Methyltransferase; EC 2.1.1.43; Transcription factor; Methyltransferase, protein lysine; DNA-binding
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription factor activity
Biological Process: ovulation; establishment and/or maintenance of chromatin architecture; ovarian follicle development; transcription, DNA-dependent; chromatin-mediated maintenance of transcription; histone H3-K4 methylation; oocyte differentiation; regulation of histone H3-K4 methylation; gene silencing
Research Articles on KMT2B
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Product Notes
The KMT2B kmt2b (Catalog #AAA388140) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KMT2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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