SDS-Page
(Recombinant DNMT3B (212-358) gel.DNMT3B (212-358) was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
DNMT3B recombinant protein
Recombinant DNMT3B (212-358) protein
Gene Names
DNMT3A; TBRS; DNMT3A2; M.HsaIIIA
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
DNMT3B; Recombinant DNMT3B (212-358) protein; DNA Methyltransferase 3 Beta; ICF1; EC 2.1.1.37; DNMT3B recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Recombinant DNMT3B (212-358) was expressed in E Colicells and is supplied in 25mM Tris pH7.4, 150mM NaCl, 5% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant DNMT3B (212-358) gel.DNMT3B (212-358) was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant DNMT3B (212-358) gel.DNMT3B (212-358) was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
Related Product Information for DNMT3B recombinant protein
Short Description: The peptide corresponding to amino acids 212-358 that contains the PWWP sequences of DNMT3B (accession number NP_715640.2) was expressed in E Coli and contains an N-terminal GST-Tag with an observed molecular weight of 42.4 kDa. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in the evaluation of CpG DNA methylation and transcriptional repression during embryonic development, genetic imprinting and X chromosome inactivation.
Background: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta)protein is a de novo DNA methyltransferase. It is involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.Trimethylation of Lys36 on histone H3 tail (H3K36me3) is associated with DNA methylation and elongation phase of transcription. PWWP domain of DNMT3B reads this epigenetic mark to guide DNA methylation.
Background: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta)protein is a de novo DNA methyltransferase. It is involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.Trimethylation of Lys36 on histone H3 tail (H3K36me3) is associated with DNA methylation and elongation phase of transcription. PWWP domain of DNMT3B reads this epigenetic mark to guide DNA methylation.
Product Categories/Family for DNMT3B recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,866 Da
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 3A isoform b
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 3 alpha
NCBI Official Symbol
DNMT3A
NCBI Official Synonym Symbols
TBRS; DNMT3A2; M.HsaIIIA
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2
Protein Family
Similar Products
Product Notes
The DNMT3B (Catalog #AAA388246) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DNMT3B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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