SDS-Page
(Recombinant KAT6A / MOZ (488-778) protein gel 10% SDS-PAGE gel with Coomassie staining MW: 35.5 kDa Purity: > 95%)
KAT6A/MOZ recombinant protein
Recombinant KAT6A/MOZ (488-778) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KAT6A / MOZ (488-778) protein gel 10% SDS-PAGE gel with Coomassie staining MW: 35.5 kDa Purity: > 95%)
SDS-Page
(Recombinant KAT6A / MOZ (488-778) protein gel 10% SDS-PAGE gel with Coomassie staining MW: 35.5 kDa Purity: > 95%)
Western Blot (WB)
(Recombinant KAT6A / MOZ (488-778) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6A / MOZ (488-778) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3 pan-acetyl antibody .)
Western Blot (WB)
(Recombinant KAT6A / MOZ (488-778) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6A / MOZ (488-778) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3 pan-acetyl antibody .)
Background: KAT6A (Lysine Acetyltransferase 6A), also called as MOZ, MYST3 or ZNF220, is a histone acetyltransferase that acetylates lysine residues in histone H3 and histone H4 (in vitro). It is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. KAT6A may act as a transcriptional coactivator for RUNX1 and RUNX2. It also acetylates p53/TP53 at 'Lys-120' and 'Lys-382' and controls its transcriptional activity via association with PML. This protein is composed of a nuclear localization domain, a double C2H2 zinc finger domain that binds to acetylated histone tails, a histone acetyl-transferase domain, a glutamate/aspartate-rich region, and a serine- and methionine-rich transactivation domain.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the MOZ, YBFR2, SAS2, TIP60 family of histone acetyltransferases. The protein is composed of a nuclear localization domain, a double C2H2 zinc finger domain that binds to acetylated histone tails, a histone acetyl-transferase domain, a glutamate/aspartate-rich region, and a serine- and methionine-rich transactivation domain. It is part of a complex that acetylates lysine-9 residues in histone 3, and in addition, it acts as a co-activator for several transcription factors. Allelic variants of this gene are associated with autosomal dominant mental retardation-32. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Uniprot Description
MYST3: Histone acetyltransferase that acetylates lysine residues in histone H3 and histone H4 (in vitro). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. May act as a transcriptional coactivator for RUNX1 and RUNX2. Chromosomal aberrations involving KAT6A may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with CREBBP; translocation t(8;22)(p11;q13) with EP300. KAT6A- CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation. A chromosomal aberration involving KAT6A is a cause of therapy-related myelodysplastic syndrome. Translocation t(2;8)(p23;p11.2) with ASXL2 generates a KAT6A-ASXL2 fusion protein. Belongs to the MYST (SAS/MOZ) family.
Protein type: Acetyltransferase; Oncoprotein; Nucleolus; EC 2.3.1.48
Chromosomal Location of Human Ortholog: 8p11
Cellular Component: nucleoplasm; Golgi apparatus; PML body; nucleolus; nucleosome; nucleus
Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; zinc ion binding; transcription coactivator activity; acetyltransferase activity; chromatin binding; transcription factor binding
Biological Process: heart morphogenesis; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; embryonic hemopoiesis; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; nucleosome assembly; protein amino acid acetylation; myeloid cell differentiation; histone acetylation; negative regulation of transcription, DNA-dependent; DNA packaging
Disease: Mental Retardation, Autosomal Dominant 32
Research Articles on KAT6A/MOZ
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Product Notes
The KAT6A/MOZ kat6a (Catalog #AAA389272) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KAT6A/MOZ, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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