SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
IL-5 active protein
Recombinant canine IL-5 protein
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
IL-5; Recombinant canine IL-5 protein; Interleukin-5; EDF; TRF; Eosinophil differentiation factor; T-cell replacing factor; IL-5 active protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
22-134aa
Sequence
VENPMNRLVA ETLTLLSTHR TWLIGDGNLM IPTPENKNHQ LCIKEVFQGI DTLKNQTAHG EAVDKLFQNL SLIKEHIERQ KKRCAGERWR VTKFLDYLQV FLGVINTEWT PES
Applicable Applications for IL-5 active protein
SDS-PAGE, Bioactivity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Canine
Tag
His-Tag
Predicted N-term
Val 22
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range <= 15ng/ml.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Biological Activity
(Canine IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range )
Biological Activity
(Canine IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range )
Related Product Information for IL-5 active protein
IL-5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant canine IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques
Product Categories/Family for IL-5 active protein
References
Martinez-Moczygemba M., et al. (2003) J Allergy Clin Immunol. 112:653-665. Milburn MV., et al. (1993) Nature. 363:172-176.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
13.9kDa (119aa)
UniProt Protein Name
Interleukin-5
UniProt Gene Name
IL5
UniProt Synonym Gene Names
IL-5; TRF
Uniprot Description
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
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Product Notes
The IL-5 il5 (Catalog #AAA201298) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-134aa. AAA Biotech's IL-5 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the IL-5 il5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VENPMNRLVA ETLTLLSTHR TWLIGDGNLM IPTPENKNHQ LCIKEVFQGI DTLKNQTAHG EAVDKLFQNL SLIKEHIERQ KKRCAGERWR VTKFLDYLQV FLGVINTEWT PESPrecautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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