IL-10 R alpha active protein

Recombinant IL-10 R alpha

Cat. Num. AAA201175

• Gene Names: IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE.
• Synonyms: IL-10 R alpha; Recombinant IL-10 R alpha; IL10RA; Interleukin-10 receptor subunit alpha; CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; Interleukin-10receptor subunit 1; IL-10R subunit 1; IL-10 R alpha active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS - PAGE.
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol.
• Concentration: 0.2mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 22-235aa
• Sequence: HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSYDLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILGKIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS CVQVKPSVAS RSNKGMWSKEECISLTRQYF TVTN
• Sequence Length: 578

• Applicable Applications for IL-10 R alpha active protein: SDS-PAGE; Bioactivity
• Tag: His-Tag
• Biological Activity: Measured by its ability to inhibit proliferation using MC/9 mouse mast cells. The ED50 for this effect is <= 300 ng/ml with Human IL-10.
• Predicted nterm: His 22
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C.Avoidrepeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing conditionandvisualized by coomassie blue stain)

Activity

(Human IL-10 R alpha inhibits Human IL-10 induced cell proliferation in the MC/9 mouse mast cells. The ED50 range is )

Related Product Information for IL-10 R alpha active protein

IL-10 R alpha, also known as Interleukin-10 receptor subunit alpha, is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. It has been shown to interact with Interleukin 10 and Janus kinase 1. Recombinant human IL-10 R alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-10 R alpha active protein

Hormones & Cytokines; Immunology

References

Josephson K, et al. (2001) Acta Crystallogr D Biol Crystallogr. 57(Pt 12):1908-11.;;Usacheva A, etal. (2002) J Immunol. 169(3):1302-8.

NCBI and Uniprot Product Information

• NCBI GI #: 222136575
• NCBI GeneID: 3587
• NCBI Accession #: NP_001549.2
• NCBI GenBank Nucleotide #: NP_001549.2
• UniProt Accession #: Q13651
• Molecular Weight: 25.2kDa (220aa)
• NCBI Official Full Name: interleukin-10 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 10 receptor subunit alpha
• NCBI Official Symbol: IL10RA
• NCBI Official Synonym Symbols: CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
• NCBI Protein Information: interleukin-10 receptor subunit alpha
• UniProt Protein Name: Interleukin-10 receptor subunit alpha
• UniProt Gene Name: IL10RA
• UniProt Synonym Gene Names: IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1
• UniProt Entry Name: I10R1_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

IL10RA: a receptor for interleukin 10 structurally related to interferon receptors. Mediates the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: plasma membrane; integral to membrane

Molecular Function: signal transducer activity; protein binding; interleukin-10 binding; interleukin-10 receptor activity; receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide

Disease: Inflammatory Bowel Disease 28, Autosomal Recessive