Goat RDH11 (Retinol dehydrogenase 11) ELISA Kit | RDH11 elisa kit

Human RDH11 (Retinol dehydrogenase 11) ELISA Kit

Cat. Num. AAA7612179

• Gene Names: RDH11; MDT1; CGI82; PSDR1; RALR1; SCALD; ARSDR1; HCBP12; RDJCSS; SDR7C1
• Reactivity: Goat
• Synonyms: RDH11 (Retinol dehydrogenase 11); Human RDH11 (Retinol dehydrogenase 11) ELISA Kit; RDH11; Retinol dehydrogenase 11; Androgen-regulated short-chain dehydrogenase/reductase 1; HCV core-binding protein HCBP12; Prostate short-chain dehydrogenase/reductase 1; Retinal reductase 1; RalR1; ARSDR1; PSDR1; SDR7C1; CGI-82; Short chain dehydrogenase/reductase family 7C member 1; RDH11 elisa kit

• Reactivity: Goat
• Specificity: This assay has high sensitivity and excellent specificity for detection of RDH11. No significant cross-reactivity or interference between RDH11 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RDH11 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 166795268
• NCBI GeneID: 51109
• NCBI Accession #: NP_057110.3
• NCBI GenBank Nucleotide #: NM_016026.3
• UniProt Accession #: Q8TC12; Q0QD40; Q6IAH5; Q9NRW0; Q9Y391; A6NDK3; A8K062; B2RB26; B4DDW0
• Molecular Weight: 27,402 Da
• NCBI Official Full Name: retinol dehydrogenase 11 isoform 1
• NCBI Official Synonym Full Names: retinol dehydrogenase 11 (all-trans/9-cis/11-cis)
• NCBI Official Symbol: RDH11
• NCBI Official Synonym Symbols: MDT1; CGI82; PSDR1; RALR1; SCALD; ARSDR1; HCBP12; RDJCSS; SDR7C1
• NCBI Protein Information: retinol dehydrogenase 11; retinal reductase 1; HCV core-binding protein HCBP12; prostate short-chain dehydrogenase reductase 1; prostate short-chain dehydrogenase/reductase 1; short chain dehydrogenase/reductase family 7C, member 1; androgen-regulated sho
• UniProt Protein Name: Retinol dehydrogenase 11
• Protein Family: Retinol dehydrogenase
• UniProt Gene Name: RDH11
• UniProt Synonym Gene Names: ARSDR1; PSDR1; RalR1
• UniProt Entry Name: RDH11_HUMAN

NCBI Description

The protein encoded by this gene is an NADPH-dependent retinal reductase and a short-chain dehydrogenase/reductase. The encoded protein has no steroid dehydrogenase activity. [provided by RefSeq, Nov 2011]

Uniprot Description

RDH11: Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent retinal reductase. Displays high activity towards 9-cis and all-trans-retinol. Also involved in the metabolism of short-chain aldehydes. No steroid dehydrogenase activity detected. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.300

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: endoplasmic reticulum membrane; photoreceptor inner segment; integral to membrane; intracellular

Molecular Function: retinol dehydrogenase activity

Biological Process: phototransduction, visible light; retinal metabolic process; retinol metabolic process; adaptation of rhodopsin mediated signaling; retinoid metabolic process

Disease: Retinal Dystrophy, Juvenile Cataracts, And Short Stature Syndrome