Rabbit anti-Human Leukotriene A4 Hydrolase Monoclonal Antibody | anti-LTA4H antibody

Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal

Cat. Num. AAA8102069

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A
• Synonyms: Leukotriene A4 Hydrolase; Monoclonal Antibody; Recombinant Anti-Leukotriene A4 Hydrolase Antibody; Rabbit Monoclonal; Leukotriene A4 Hydrolase/LTA4H Antibody; Rabbit MAb; leukotriene A4 hydrolase; Anti-LAT4 Antibody; anti-LTA4H antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 201
• Specificity: Human Leukotriene A4 Hydrolase/LTA4H.; No cross-reactivity in ELISA with Mouse LTA4H
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LTA4H antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; ICC/IF: 1:100-1:500; IP: 1-2uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Leukotriene A4 Hydrolase/LTA4H protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Leukotriene A4 Hydrolase/LTA4H (rh Leukotriene A4 Hydrolase/LTA4H).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LTA4H monoclonal antibody (1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Western Blot (WB)

(Anti-LTA4H rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:69 kDaObserved band size:69 kDa)

Immunoprecipitation (IP)

(LTA4H was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-LTA4H rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)

Related Product Information for anti-LTA4H antibody

Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H, and LTA4, is a cytoplasm protein that belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may, in turn, be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the pro-inflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.

References

Mancini, JA. et al., 1995, Eur. J. Biochem. 231: 65-71. Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-73. Rudberg, PC. et al., 2004, J. Biol. Chem. 279: 27376-82. Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-6.

NCBI and Uniprot Product Information

• NCBI GI #: 4505029
• NCBI GeneID: 4048
• NCBI Accession #: NP_000886.1
• NCBI GenBank Nucleotide #: NM_000895.2
• UniProt Accession #: P09960; Q6IAT6; Q9UCT7; B4DNQ9; F8VV40
• Molecular Weight: 66,852 Da
• NCBI Official Full Name: leukotriene A-4 hydrolase isoform 1
• NCBI Official Synonym Full Names: leukotriene A4 hydrolase
• NCBI Official Symbol: LTA4H
• NCBI Protein Information: leukotriene A-4 hydrolase
• UniProt Protein Name: Leukotriene A-4 hydrolase
• Protein Family: Leukotriene A-4 hydrolase
• UniProt Gene Name: LTA4H
• UniProt Synonym Gene Names: LTA4; LTA-4 hydrolase
• UniProt Entry Name: LKHA4_HUMAN

Uniprot Description

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.3.2.6; Hydrolase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: peptidase activity; leukotriene-A4 hydrolase activity; metallopeptidase activity; zinc ion binding; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; peptide catabolic process; arachidonic acid metabolic process; leukotriene metabolic process; proteolysis; inflammatory response