SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ARPP19 recombinant protein
ARPP19 Protein
Applications
Western Blot
Synonyms
ARPP19; ARPP19 Protein; ARPP-16; ARPP-19; ARPP16; ENSAL; ARPP19 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM Tris -HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for ARPP19 recombinant protein
Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for ARPP19 recombinant protein
Full-length recombinant human ARPP19 was expressed in E Coli cells using an N-terminal GST tag. The gene accession number is NM_006628.
ARPP19, cAMP-regulated phosphoprotein 19, is specifically phosphorylated at Ser-62 during mitosis by MASTL, also known as the Greatwall kinase. The phos-phorylation of ARPP19 leads to inactivation of PP2A through its interaction with PR55-delta, which is essential in maintaining the activity of CDK1/cyclin B1 and promoting mitotic G2/M transition (1). Significant reduction of ARPP-19 was observed in brains of Alzheimer's disease and Down syndrome (2). ARPP-19 was also found to contribute to tumor growth in hepatocellular carcinoma and papillary carcinoma (3).
ARPP19, cAMP-regulated phosphoprotein 19, is specifically phosphorylated at Ser-62 during mitosis by MASTL, also known as the Greatwall kinase. The phos-phorylation of ARPP19 leads to inactivation of PP2A through its interaction with PR55-delta, which is essential in maintaining the activity of CDK1/cyclin B1 and promoting mitotic G2/M transition (1). Significant reduction of ARPP-19 was observed in brains of Alzheimer's disease and Down syndrome (2). ARPP-19 was also found to contribute to tumor growth in hepatocellular carcinoma and papillary carcinoma (3).
Product Categories/Family for ARPP19 recombinant protein
References
1. Gharbi-Ayachi, A., et al. The substrate of Greatwall kinase, Arpp19, controls mitosis by inhibiting protein phosphatase 2A. Science. 330(6011): 1673-1677, 2010.
2. Kim, S. H., et al. Decreased levels of ARPP-19 and PKA in brains of Down syndrome and Alzheimer's disease. J Neural Transm Suppl. 61: 263-272, 2001.
3. Song, H., et al. Increased ARPP-19 expression is associated with hepatocellular carcinoma. Int J Mol Sci. 16(1): 178-192, 2014.
2. Kim, S. H., et al. Decreased levels of ARPP-19 and PKA in brains of Down syndrome and Alzheimer's disease. J Neural Transm Suppl. 61: 263-272, 2001.
3. Song, H., et al. Increased ARPP-19 expression is associated with hepatocellular carcinoma. Int J Mol Sci. 16(1): 178-192, 2014.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46kDa
Protein Family
Similar Products
Product Notes
The ARPP19 (Catalog #AAA516576) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ARPP19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ARPP19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARPP19, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
