Typical Testing Data/Standard Curve (for reference only)
Cytochrome Oxidase Assay Kit
Cytochrome Oxidase Activity Colorimetric Assay Kit
Synonyms
Cytochrome Oxidase; Cytochrome Oxidase Activity Colorimetric Assay Kit; Cytochrome Oxidase assay kit
Samples
Purified mitochondria
Cells/Tissue extracts
Cells/Tissue extracts
Features & Benefits
Simple & Rapid
High-throughput adaptable
The kit provides unique formulation of buffers & reagents to measure cytochrome oxidase activity
High-throughput adaptable
The kit provides unique formulation of buffers & reagents to measure cytochrome oxidase activity
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cytochrome Oxidase assay kit
Product Information: Cytochrome Oxidase Activity Colorimetric Assay Kit: Rapidly measures cytochrome oxidase activity from purified mitochondria or cell/tissue extracts. Simple & HTS. 100 assays
Background: Cytochrome c Oxidase (EC 1.9.3.1) or Complex IV is the fourth complex of the Electron Transport Chain located in the mitochondrial (or bacterial) membrane. It provides energy to the cell by coupling electron transport through the Cytochrome c chain with the process of oxidative phosphorylation. Complex IV contains 13 different subunits encoded by both mitochondrial DNA and nuclear DNA. It receives an electron from each of the four Cytochrome c molecules, and transfers it to one oxygen molecule, converting it into two molecules of water. In this process, it also binds to four proton molecules and translocates them across the membrane to establish electrochemical gradient, which is utilized for the synthesis of ATP. Cytochrome Oxidase Activity Assay Kit is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.
Summary: Simple and rapid measurement of cytochrome oxidase enzymatic activity in 96-well plate format.
Useful for mitochondrial respiration studies, assembly of the complexes, mitochondria outer membrane integrity.
Background: Cytochrome c Oxidase (EC 1.9.3.1) or Complex IV is the fourth complex of the Electron Transport Chain located in the mitochondrial (or bacterial) membrane. It provides energy to the cell by coupling electron transport through the Cytochrome c chain with the process of oxidative phosphorylation. Complex IV contains 13 different subunits encoded by both mitochondrial DNA and nuclear DNA. It receives an electron from each of the four Cytochrome c molecules, and transfers it to one oxygen molecule, converting it into two molecules of water. In this process, it also binds to four proton molecules and translocates them across the membrane to establish electrochemical gradient, which is utilized for the synthesis of ATP. Cytochrome Oxidase Activity Assay Kit is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.
Summary: Simple and rapid measurement of cytochrome oxidase enzymatic activity in 96-well plate format.
Useful for mitochondrial respiration studies, assembly of the complexes, mitochondria outer membrane integrity.
Product Categories/Family for Cytochrome Oxidase assay kit
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Product Notes
The Cytochrome Oxidase (Catalog #AAA841652) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cytochrome Oxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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