Typical Testing Data/Standard Curve (for reference only)
Mitochondria Isolation Kit Assay Kit
Yeast Mitochondria Isolation Kit
Synonyms
Mitochondria Isolation Kit; Yeast Mitochondria Isolation Kit; Mitochondria Isolation Kit assay kit
Samples
Yeast cells culture.
Features & Benefits
Simple & convenient protocol
The kit provides unique formulations of ready-to-use buffers and reagents to isolate intact mitochondria
The kit provides unique formulations of ready-to-use buffers and reagents to isolate intact mitochondria
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Mitochondria Isolation Kit assay kit
Product Information: Yeast Mitochondria Isolation Kit: Highly efficient kit to isolate intact yeast mitochondria. Can be used to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA & mtRNA & for protein profiling. 50 Assays
Background: Mitochondria are the power house of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria is a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. MyBioSource's Yeast Mitochondria Isolation kit will enable fast and easy purification of mitochondria from yeast cells, utilizing yeast cell wall lysis and homogenization. This Mitochondria Isolation Kit is tested for Pichia pastoris & Saccharomyces cerevisiae & can be used to isolate mitochondria from other yeast. Under fermentable media, the yield is ~150-200 ug of mitochondria & under non-fermentable media (e.g. Glycerol) ~200-250 ug of mitochondria from a culture of OD ~20.
Summary: Application
Isolation of highly pure intact mitochondria from yeast cells.
Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.
Western blot and ELISA
Background: Mitochondria are the power house of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria is a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. MyBioSource's Yeast Mitochondria Isolation kit will enable fast and easy purification of mitochondria from yeast cells, utilizing yeast cell wall lysis and homogenization. This Mitochondria Isolation Kit is tested for Pichia pastoris & Saccharomyces cerevisiae & can be used to isolate mitochondria from other yeast. Under fermentable media, the yield is ~150-200 ug of mitochondria & under non-fermentable media (e.g. Glycerol) ~200-250 ug of mitochondria from a culture of OD ~20.
Summary: Application
Isolation of highly pure intact mitochondria from yeast cells.
Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.
Western blot and ELISA
Product Categories/Family for Mitochondria Isolation Kit assay kit
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Product Notes
The Mitochondria Isolation Kit (Catalog #AAA841667) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mitochondria Isolation Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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