Typical Testing Data/Standard Curve (for reference only)
Cell-Mediated Cytotoxicity (7-AAD/CFSE) Assay Kit
Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD / CFSE)
Synonyms
Cell-Mediated Cytotoxicity (7-AAD/CFSE); Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD / CFSE); Cell-Mediated Cytotoxicity (7-AAD/CFSE) assay kit
Application Notes
Measurement of cell cytotoxicity in response to drug or toxin treatment
Quantification of the cytotoxic effect of immune effector cells on target cells
Assessment of physiological mediators and antibodies that affect cell cytotoxicity
Quantification of the cytotoxic effect of immune effector cells on target cells
Assessment of physiological mediators and antibodies that affect cell cytotoxicity
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cell-Mediated Cytotoxicity (7-AAD/CFSE) assay kit
Background/Introduction: Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.
Product Categories/Family for Cell-Mediated Cytotoxicity (7-AAD/CFSE) assay kit
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Product Notes
The Cell-Mediated Cytotoxicity (7-AAD/CFSE) (Catalog #AAA841493) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of cell cytotoxicity in response to drug or toxin treatment Quantification of the cytotoxic effect of immune effector cells on target cells Assessment of physiological mediators and antibodies that affect cell cytotoxicity. Researchers should empirically determine the suitability of the Cell-Mediated Cytotoxicity (7-AAD/CFSE) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cell-Mediated Cytotoxicity (7-AAD/CFSE), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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