Typical Testing Data/Standard Curve (for reference only)
Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 Assay Kit
Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1
Synonyms
Ready-to-use Cell Proliferation Colorimetric Reagent; WST-1; WST-1 assay kit
Samples
Cell cultures (adherent and suspension)
Features & Benefits
Simple one-step procedure; takes less than 4 hours
Fast and convenient
The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.
Fast and convenient
The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 assay kit
Product Information: Ready-to-Use Cell Proliferation Reagent, WST-1: Measure Cell Proliferation in response to growth factors, cytokines, mitogens and nutrients etc. in the same microtiter plate. No need to wash, harvest & solubilize cells. 2500 Assays.
Background: The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
Summary: Detection method- Absorbance 440 nm
Species reactivity- Mammalian
Applications- Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators and antibodies that inhibit cell growth.
Background: The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
Summary: Detection method- Absorbance 440 nm
Species reactivity- Mammalian
Applications- Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators and antibodies that inhibit cell growth.
Product Categories/Family for Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 assay kit
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Product Notes
The Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 (Catalog #AAA841449) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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