TRAIL/TNFSF10 Recombinant Protein | TNFSF10 recombinant protein

TRAIL/TNFSF10 Protein, Mouse, Recombinant (aa 118-291, His Tag)

Cat. Num. AAA8122577

• Gene Names: Tnfsf10; TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TRAIL/TNFSF10; TRAIL/TNFSF10 Protein; Mouse; Recombinant (aa 118-291; His Tag); Mouse TNFSF10/TRAIL/APO-2L Protein (aa 118-291; A330042I21Rik Protein; AI448571 Protein; APO-2L Protein; Ly81 Protein; TL2 Protein; Trail Protein; tumor necrosis factor (ligand) superfamily; member 10; TNFSF10 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Liquid. Supplied as sterile PBS, pH7.5. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro118-Asn291

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse TNFSF10 (P50592)(Pro 118-Asn 291) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; It is shipped out with blue ice.

SDS-Page

Related Product Information for TNFSF10 recombinant protein

Background: Tumor necrosis factor ligand superfamily member 10 (TNFSF10), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF10/Apo-2L/CD253 functions as a ligand that induces the process of cell death called apoptosis. TNFSF10/TRAIL shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF10/CD253 is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF10/Apo-2L/CD253/TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF10/TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for TNFSF10 recombinant protein

Cancer Drug Targets; Death Receptor Signaling

References

Song C, et al. (2005) TRAIL (CD253), a new member of the TNF superfamily. J Biol Regul Homeost Agents. 19(1-2): 73-7.
Kuribayashi K, et al. (2008) TNFSF10 (TRAIL), a p53 target gene that mediates p53-dependent cell death. Cancer Biol Ther. 7(12): 2034-8.
Wiley SR, et al. (1995) Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 3(6): 673-82.

NCBI and Uniprot Product Information

• NCBI GI #: 6678431
• NCBI GeneID: 22035
• NCBI Accession #: NP_033451.1
• NCBI GenBank Nucleotide #: NM_009425.2
• UniProt Accession #: P50592
• Molecular Weight: 33,477 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: Tnfsf10
• NCBI Official Synonym Symbols: TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand; TRAIL/APO2L
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf10
• UniProt Synonym Gene Names: Trail; Protein TRAIL
• UniProt Entry Name: TNF10_MOUSE

Uniprot Description

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; immune response