Testing Data
General AMP Assay Kit
AMP Colorimetric Assay Kit
Reactivity
General
Synonyms
AMP; AMP Colorimetric Assay Kit; AMP Assay Kit; AMP Colorimetric Kit; Adenosine Monophosphate Assay; Adenosine Monophosphate Kit; Adenosine Monophosphate Colorimetric Assay; AMP Colorimetric Assay; Adenosine Monophosphate; AMP Assay; Adenosine Monophosphate Colorimetric Kit; AMP assay kit
Reactivity
General
Samples
Adherent or suspension Cells: e.g. HeLa, HEK239, Jurkat cells
Tissuelysates: e.g. Liver, Kidney, etc
Tissuelysates: e.g. Liver, Kidney, etc
Detection Method
Absorbance: OD 570nm
Features & Benefits
Study the regulation of AMPK by AMP, Mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc.
Measurement of AMP level in various cell or tissue types
Measurement of AMP level in various cell or tissue types
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for AMP assay kit
Adenosine Monophosphate (AMP), also known as 5'-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories. AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 uM in various sample types.
Product Categories/Family for AMP assay kit
Similar Products
Product Notes
The General AMP (Catalog #AAA849611) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849611 Assay Kit recognizes General AMP. It is sometimes possible for the material contained within the vial of "AMP, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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