Typical Standard Curve/Testing Data
Sirtuin Assay Kit
Sirtuin Activity Assay Kit (Fluorometric)
Synonyms
Sirtuin; Sirtuin Activity Assay Kit (Fluorometric); Sirt Activity Assay Kit (Fluorometric); Sirtuin assay kit
Application Notes
Detection of Sirtuin Activity in variety of samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Sirtuin assay kit
Background/Introduction: Sirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. They are associated with aging, cellular protection, sugar metabolism and cell cycle regulation. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP- ribosyltransferase activity. In Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. HDAC's also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC's in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay is 0.06 uU of recombinant human SIRT6.
Product Categories/Family for Sirtuin assay kit
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Product Notes
The Sirtuin (Catalog #AAA841650) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Detection of Sirtuin Activity in variety of samples. Researchers should empirically determine the suitability of the Sirtuin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sirtuin, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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