Typical Testing Data/Standard Curve (for reference only)
Beta-Lactamase Assay Kit | blaC assay kit
Beta-Lactamase Activity Colorimetric Assay Kit
Synonyms
Beta-Lactamase; Beta-Lactamase Activity Colorimetric Assay Kit; blaC assay kit
Sequence Length
307
Application Notes
Measurement of beta-Lactamase activity in various biological samples
Analysis of beta-Lactamase activity in pathological conditions
Analysis of beta-Lactamase activity in pathological conditions
Samples
Serum, urine, saliva from mammals infected with betaL-secreting bacteria
Food (e.g. milk)
Fermentation media, bacterial cultures, etc.
Food (e.g. milk)
Fermentation media, bacterial cultures, etc.
Detection Method
Absorbance (490 nm)
Features & Benefits
Rapid, simple & sensitive
The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.
The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for blaC assay kit
Product Information: Beta-Lactamase Activity Colorimetric Assay Kit: Rapidly measures beta lactamase activity in food, fermentation media, bacterial cultures, serum, urine, saliva etc. from mammals. 100 assays.
Background: Beta-Lactamases (betaLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. betaLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as beta-lactams. Antibiotics containing beta-lactam rings (i.e. penicillin, cephalosporin, monobactam, carbapenem) are highly susceptible to be hydrolyzed via enzymatic activity, which deactivates their antibiotic potency. betaLs have become a significant clinical threat due to the alarming number of cases of bacterial strains showing beta-lactam antibiotic resistance. MyBioSource's Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD 490 nm), which is directly proportional to the amount of betaL activity. The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.
Summary: Detection method: Absorbance (490 nm)
Application: - Measurement of beta-Lactamase activity in various biological samples - Analysis of beta-Lactamase activity in pathological conditions
Background: Beta-Lactamases (betaLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. betaLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as beta-lactams. Antibiotics containing beta-lactam rings (i.e. penicillin, cephalosporin, monobactam, carbapenem) are highly susceptible to be hydrolyzed via enzymatic activity, which deactivates their antibiotic potency. betaLs have become a significant clinical threat due to the alarming number of cases of bacterial strains showing beta-lactam antibiotic resistance. MyBioSource's Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD 490 nm), which is directly proportional to the amount of betaL activity. The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.
Summary: Detection method: Absorbance (490 nm)
Application: - Measurement of beta-Lactamase activity in various biological samples - Analysis of beta-Lactamase activity in pathological conditions
Product Categories/Family for blaC assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,568 Da
NCBI Official Full Name
Beta-lactamase
NCBI Official Symbol
blaC
NCBI Protein Information
beta-lactamase
UniProt Protein Name
Beta-lactamase
Protein Family
UniProt Gene Name
blaC
UniProt Entry Name
BLAC_MYCTU
Uniprot Description
Extended spectrum beta-lactamase (ESBL) that inactivates beta-lactam antibiotics by hydrolyzing the amide group of the beta-lactam ring. Displays high levels of penicillinase and cephalosporinase activity as well as measurable activity with carbapenems, including imipenem and meropenem. Plays a primary role in the intrinsic resistance of M.tuberculosis to beta-lactam antibiotics.
Research Articles on blaC
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Product Notes
The blaC blac (Catalog #AAA841861) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of beta-Lactamase activity in various biological samples Analysis of beta-Lactamase activity in pathological conditions. Researchers should empirically determine the suitability of the blaC blac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-Lactamase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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