Testing Data
Mammalian Glutaminase (GLS) Assay Kit
PicoProbe Glutaminase (GLS) Activity Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Glutaminase (GLS); PicoProbe Glutaminase (GLS) Activity Assay Kit (Fluorometric); GLS2; GLS; Glutaminase; GLS1; Glutaminase (GLS) assay kit
Reactivity
Mammalian
Samples
Tissue lysate (e.g. Liver tissue), Cell lysate, Recombinant enzyme, Purified protein
Detection Method
Fluorometric: Ex/Em 535/587nm
Features & Benefits
Validated with relevant biological samples
Includes human GLS as positive control
Sensitive, specific assay for detection of glutaminase activity in biological samples
Measurement of Glutaminase activity in cell and tissue lysates using a 96-well plate format
Includes human GLS as positive control
Sensitive, specific assay for detection of glutaminase activity in biological samples
Measurement of Glutaminase activity in cell and tissue lysates using a 96-well plate format
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Glutaminase (GLS) assay kit
Glutaminase (EC 3.5.1.2) is a mitochondrial enzyme that hydrolyzes glutamine producing glutamate and ammonia. GLS is one of the two key enzymes that are responsible for glutamine homeostasis, the other being glutamine synthetase. Since glutamine is a major metabolic substrate involved in gluconeogenesis, its homeostasis is tightly controlled. Glutaminase has tissue-specific roles in multiple organs, with the isoform GLS1 being expressed in kidneys and brain, and GLS2 being expressed in the liver. It is involved in maintenance of acid-base homeostasis by producing ammonia during renal acidosis in kidneys; and regulation of glutamate -which acts as a neurotransmitter- in the brain. On the other hand, GLS 2 regulates the generation of ammonia in liver by producing urea. Research shows GLS increased activity in individuals facing starvation, diabetes and high protein diets. PicoProbe Glutaminase Activity Assay Kit is a simple plate-based fluorometric assay for measuring glutaminase activity in biological samples. GLS hydrolyzes glutamine forming glutamate and ammonia. Glutamate, in the presence of a developer and enzyme mix converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. The assay can detect as low as 2 uU of glutaminase in biological samples.
Product Categories/Family for Glutaminase (GLS) assay kit
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Product Notes
The Mammalian Glutaminase (GLS) (Catalog #AAA849677) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849677 Assay Kit recognizes Mammalian Glutaminase (GLS). It is sometimes possible for the material contained within the vial of "Glutaminase (GLS), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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