MTSS1 recombinant protein

Recombinant Human MTSS1 Protein (aa1-250, His & MBP tag)

Cat. Num. AAA2545413

• Gene Names: MTSS1; MIM; MIMA; MIMB
• Purity: > 80 % as determined by reducing SDS-PAGE.
• Synonyms: MTSS1; Recombinant Human MTSS1 Protein (aa1-250; His & MBP tag); DKFZp781P2223; FLJ44694; KIAA0429; MIM; MIMA; MIMB; MTSS1 recombinant protein

• Host: E.coli
• Purity/Purification: > 80 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.5, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence Positions: Met 1-Ser 250

• Application Notes: The recombinant human MTSS1/MBP fusion protein consists of 647 amino acids and has a calculated molecular mass of 71.8 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• Species: Human
• Tag: N-His & MBP
• Reconstitution: It is recommended that sterile water (80uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for MTSS1 recombinant protein

Background: MTSS1 (Metastasis suppressor 1), also known as Missing in metastasis (MIM), is a tissue-specific regulator of plasma membrane dynamics. MTSS1 is well described for its function as a metastasis suppressor gene and is expressed in a variety of tissues. MTSS1 might be involved in shaping neuronal membranes in vivo. MTSS1 deforms phosphoinositide-rich membranes through its I-BAR domain and interacts with actin monomers through its WH2 domain. MTSS1/MIM was first identified as a metastasis suppressor missing in metastatic bladder carcinoma cell lines. MTSS1 is a prognostic indicator of disease-free survival in breast cancer patients and demonstrates the ability to play a role in governing the metastatic nature of breast cancer cells. MTSS1 may serve as a useful biomarker for the prediction of outcome of gastric cancer. The down-regulation of MTSS1 that may be caused by DNA methylation was also observed in many other type s of cancer.Recent work proposed that MIM also potentiates Sonic hedgehog (Shh)-induced gene expression. MTSS1 as a multiple functional molecular player and has an important role in development, carcinogenesis and metastasis.

Description: A DNA sequence encoding the human MTSS1 (EAW92073.1) N-terminal fragment (Met 1-Ser 250) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 119612479
• NCBI GeneID: 9788
• Molecular Weight: Mol_Mass: 71.8 kDa; AP_Mol_Mass: 66 kDa
• NCBI Official Full Name: metastasis suppressor 1, isoform CRA_e
• NCBI Official Synonym Full Names: MTSS1, I-BAR domain containing
• NCBI Official Symbol: MTSS1
• NCBI Official Synonym Symbols: MIM; MIMA; MIMB
• NCBI Protein Information: metastasis suppressor protein 1
• UniProt Protein Name: Metastasis suppressor protein 1
• Protein Family: MTSS1-like protein
• UniProt Gene Name: MTSS1
• UniProt Synonym Gene Names: KIAA0429; MIM

Uniprot Description

MTSS1: an intracellular protein that binds to actin and cortactin, and possesses tumor suppressor activity. Binds to the cytoplasmic domain of receptor protein tyrosine phosphatase delta. Senses and induces curved membranous curvatures important for cellular polarization, mobilization and endocytosis. May be related to cancer progression or tumor metastasis in a variety of organ sites. Its expression is down-regulated by MiR-135a. Downregulated in prostate cancer. Expressed in many tissues, including spleen, thymus, prostate, testis, uterus, colon, and peripheral blood. Belongs to the MTSS1 family. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: actin cytoskeleton; cytoplasm; endocytic vesicle

Molecular Function: actin monomer binding; identical protein binding; protein binding; receptor binding

Biological Process: actin cytoskeleton organization and biogenesis; negative regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway