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Western Blot (WB)
(Western blot analysis of extracts from various cell lines using eIF4G Rabbit mAb.)
Rabbit Eukaryotic Translation Initiation Factor 4G Monoclonal Antibody
Eukaryotic Translation Initiation Factor 4G (eIF4G)
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
SupernatantSupernatant
Synonyms
Eukaryotic Translation Initiation Factor 4G; Monoclonal Antibody; Eukaryotic Translation Initiation Factor 4G (eIF4G); Anti -Eukaryotic Translation Initiation Factor 4G (eIF4G); anti-Eukaryotic Translation Initiation Factor 4G antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
7H21
Specificity
Detects endogenous levels of total human eIF4G protein. Species Crossreactivity: Monkey, mouse and rat.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Applicable Applications for anti-Eukaryotic Translation Initiation Factor 4G antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry.
Immunogen
Synthetic peptide (KLH-coupled) derived from the sequence around Gly188 of human eIF4G.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using eIF4G Rabbit mAb.)
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using eIF4G Rabbit mAb.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human glioblastoma using eIF4G Rabbit mAb.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human glioblastoma using eIF4G Rabbit mAb.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF4G Rabbit mAb.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF4G Rabbit mAb.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using eIF4G Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using eIF4G Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using eIF4G Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using eIF4G Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Related Product Information for anti-Eukaryotic Translation Initiation Factor 4G antibody
The initiation of translation is an important biological event, and a variety of factors contribute to this process. The eIF4 family of translation initiation factors bind to the 5' m7GTP mRNA cap and function to unwind the secondary structure of mRNA (1, 2). The amino-terminal portion of eIF4G physically associates with eIF4E and stimulates the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A (4), serving as an adaptor molecule in the eIF4 complex. Moreover, eIF4G also plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6).
Product Categories/Family for anti-Eukaryotic Translation Initiation Factor 4G antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
eukaryotic translation initiation factor 4G
Protein Family
Similar Products
Product Notes
The Eukaryotic Translation Initiation Factor 4G (Catalog #AAA601812) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eukaryotic Translation Initiation Factor 4G (eIF4G) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Eukaryotic Translation Initiation Factor 4G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Researchers should empirically determine the suitability of the Eukaryotic Translation Initiation Factor 4G for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eukaryotic Translation Initiation Factor 4G, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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