Western Blot (WB)
(Western blot analysis of extracts of HeLacell line, using EIF4G1antibody.)
Rabbit eIF4G1 Antibody | anti-eIF4G1 antibody
eIF4G1 Antibody
Gene Names
EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
eIF4G1; eIF4G1 Antibody; DKFZp686A1451; EIF4F; EIF4G; p220; anti-eIF4G1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
1404
Applicable Applications for anti-eIF4G1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:20-1:50
IHC: 1:50-1:100
IF: 1:20-1:50
Immunogen
Fusion protein of human EIF4G1
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of HeLacell line, using EIF4G1antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLacell line, using EIF4G1antibody.)
Related Product Information for anti-eIF4G1 antibody
The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and unwind the mRNA secondary structure (1, 2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5, 6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7, 8).
Product Categories/Family for anti-eIF4G1 antibody
References
1. Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398. 2. Morley, S.J. et al. (1997) RNA 3, 1085-1104. 3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680. 4. De Gregorio, E. et al. (1998) RNA 4, 828-836. 5. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382. 6. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136. 7. Henis-Korenblit, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 5400-5405. 8. Nevins, T.A. et al. (2003) J. Biol. Chem. 278, 3572-3579.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
176kDa
NCBI Official Full Name
eukaryotic translation initiation factor 4 gamma 1 isoform 4
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4 gamma 1
NCBI Official Symbol
EIF4G1
NCBI Official Synonym Symbols
P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
NCBI Protein Information
eukaryotic translation initiation factor 4 gamma 1
UniProt Protein Name
Eukaryotic translation initiation factor 4 gamma 1
Protein Family
UniProt Gene Name
EIF4G1
UniProt Synonym Gene Names
EIF4F; EIF4G; EIF4GI; eIF-4-gamma 1; eIF-4G 1; eIF-4G1
NCBI Description
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]
Uniprot Description
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
Research Articles on eIF4G1
Similar Products
Product Notes
The eIF4G1 eif4g1 (Catalog #AAA8500674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eIF4G1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eIF4G1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:20-1:50. Researchers should empirically determine the suitability of the eIF4G1 eif4g1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4G1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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