Western Blot (WB)
(Western blot analysis of extracts from HT29 (human) and AR42J (mouse) cells, untreated or thapsigargin-treated (300 nM, 30 min), using MBS632270 (upper) or eIF2alpha Antibody P9108-48B (lower).)
Rabbit Eukaryotic Translation Initiation Factor 2, alpha Monoclonal Antibody | anti-EIF2S1 antibody
Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)
Ascites
Ascites
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:50 IHC protocol: Unmasking buffer/Antibody diluent
Citrate/TBST-5%NGS
Western Blot (WB)
(Western blot analysis of extracts from HT29 (human) and AR42J (mouse) cells, untreated or thapsigargin-treated (300 nM, 30 min), using MBS632270 (upper) or eIF2alpha Antibody P9108-48B (lower).)
Western Blot (WB)
(Western blot analysis of extracts from HT29 (human) and AR42J (mouse) cells, untreated or thapsigargin-treated (300 nM, 30 min), using MBS632270 (upper) or eIF2alpha Antibody P9108-48B (lower).)
Western Blot (WB)
(Western blot analysis of extracts from HeLa and THP-1 cells untreated or phosphatase treated, using MBS632270)
Western Blot (WB)
(Western blot analysis of extracts from HeLa and THP-1 cells untreated or phosphatase treated, using MBS632270)
Western Blot (WB)
(Western blot analysis of immunoprecipitates from PC12 cells, using E8949-03Lane 1 is lysate control, lane 2 is the antibody alone as negative control and lane 3 is antibody immunocomplex of PC12 cells.)
Western Blot (WB)
(Western blot analysis of immunoprecipitates from PC12 cells, using E8949-03Lane 1 is lysate control, lane 2 is the antibody alone as negative control and lane 3 is antibody immunocomplex of PC12 cells.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using E8949-03Rabbit mAb.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using E8949-03Rabbit mAb.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells, serum starved vs. Thapsigargin treated (30 minutes) using MBS632270 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells, serum starved vs. Thapsigargin treated (30 minutes) using MBS632270 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
NCBI and Uniprot Product Information
NCBI Description
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Uniprot Description
eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.
Protein type: Translation; Translation initiation; RNA-binding
Chromosomal Location of Human Ortholog: 14q23.3
Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex
Molecular Function: protein binding; translation initiation factor activity; ribosome binding
Biological Process: regulation of translation initiation in response to stress; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression
Research Articles on EIF2S1
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Product Notes
The EIF2S1 eif2s1 (Catalog #AAA632270) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a) reacts with Drosophila, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Eukaryotic Translation Initiation Factor 2, alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 IHC protocol: Unmasking buffer/Antibody diluent Citrate/TBST-5%NGS. Researchers should empirically determine the suitability of the EIF2S1 eif2s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eukaryotic Translation Initiation Factor 2, alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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