Western Blot (WB)
(Detection of Human Thymosin b10 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1ug/ml of Human Thymosin b10 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated AntiSheep IgG Secondary Antibody . A specific band was detected for Thymosin ß10 at approximately 6kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. PREPARATION)
Sheep anti-Human Thymosin B10 Polyclonal Antibody
Thymosin B10
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Thymosin B10; Polyclonal Antibody; Anti -Thymosin B10; anti-Thymosin B10 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Thymosin B10.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS,.PBS with Trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-Thymosin B10 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1mg/ml
Dilution: Western Blot: 1mg/ml
Immunogen
Recombinant corresponding to human Thymosin B10 expressed in E. coli (P63313).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Human Thymosin b10 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1ug/ml of Human Thymosin b10 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated AntiSheep IgG Secondary Antibody . A specific band was detected for Thymosin ß10 at approximately 6kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. PREPARATION)
Western Blot (WB)
(Detection of Human Thymosin b10 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1ug/ml of Human Thymosin b10 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated AntiSheep IgG Secondary Antibody . A specific band was detected for Thymosin ß10 at approximately 6kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. PREPARATION)
Related Product Information for anti-Thymosin B10 antibody
Thymosin beta 10 (TB10) is a 6.5kD member of the Bthymosin family of molecules. Members of this family range from 41-44aa in length, and possess an isoelectric point that lies between pH 5.07.0. Multiple cell types produce TB10, either constitutively, or after stimulation. They include monocytes/macrophages, endothelial cells, thyroid epithelium, spermatids, fetal cerebellar Purkinje cells plus renal tubule epithelium, and multiple tumor types. TB10 is
Product Categories/Family for anti-Thymosin B10 antibody
Similar Products
Product Notes
The Thymosin B10 (Catalog #AAA6012876) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Thymosin B10 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thymosin B10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1mg/ml. Researchers should empirically determine the suitability of the Thymosin B10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thymosin B10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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