Western Blot (WB)
(Detection of Human and Mouse PEDF R/PNPLA2 by Western Blot. Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1ug/ml of MBS620634 followed by HRPconjugated AntiSheep IgG Secondary Antibody A specific band was detected for PEDF R/PNPLA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Sheep PNPLA2 Polyclonal Antibody | anti-PNPLA2 antibody
PNPLA2 (PEDFR, Patatin-like phospholipase domain containing 2, 1110001C14Rik, Adipose triglyceride lipase, ATGL, Calcium-independent phospholipase A2, desnutrin, Desnutrin, DESNUTRIN, DKFZp667M109, FP17548, IPLA2-zeta, Patatin-like phospholipase domain-co
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Immunohistochemistry: 5-15ug/ml detects PNPLA2 in cells and tissues
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant mouse and recombinant human PNPLA2 is ~1ng/well
Western Blot (WB)
(Detection of Human and Mouse PEDF R/PNPLA2 by Western Blot. Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1ug/ml of MBS620634 followed by HRPconjugated AntiSheep IgG Secondary Antibody A specific band was detected for PEDF R/PNPLA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Western Blot (WB)
(Detection of Human and Mouse PEDF R/PNPLA2 by Western Blot. Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1ug/ml of MBS620634 followed by HRPconjugated AntiSheep IgG Secondary Antibody A specific band was detected for PEDF R/PNPLA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Immunohistochemistry (IHC)
(PEDF R/PNPLA2 in Mouse Kidney. PEDF R/PNPLA2 was detected in perfusion fixed frozen sections of mouse kidney using 15ug/ml MBS620634 overnight at 4 °C. Tissue was stained with the Anti Sheep HRPDAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
Immunohistochemistry (IHC)
(PEDF R/PNPLA2 in Mouse Kidney. PEDF R/PNPLA2 was detected in perfusion fixed frozen sections of mouse kidney using 15ug/ml MBS620634 overnight at 4 °C. Tissue was stained with the Anti Sheep HRPDAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq]
Uniprot Description
PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 3.1.1.3; Lipase
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; plasma membrane; integral to membrane; lipid particle; cytosol
Molecular Function: triacylglycerol lipase activity
Biological Process: sequestering of lipid; phospholipid metabolic process; triacylglycerol catabolic process; glycerophospholipid biosynthetic process
Disease: Neutral Lipid Storage Disease With Myopathy
Research Articles on PNPLA2
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Product Notes
The PNPLA2 pnpla2 (Catalog #AAA620634) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The PNPLA2 (PEDFR, Patatin-like phospholipase domain containing 2, 1110001C14Rik, Adipose triglyceride lipase, ATGL, Calcium-independent phospholipase A2, desnutrin, Desnutrin, DESNUTRIN, DKFZp667M109, FP17548, IPLA2-zeta, Patatin-like phospholipase domain-co reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PNPLA2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Dilution: Western Blot: 1.0ug/ml Immunohistochemistry: 5-15ug/ml detects PNPLA2 in cells and tissues Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant mouse and recombinant human PNPLA2 is ~1ng/well. Researchers should empirically determine the suitability of the PNPLA2 pnpla2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNPLA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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