Typical Testing Data/Standard Curve (for reference only)
M13 Phage Titration ELISA Kit
M13 Phage Titration ELISA Kit
Applications
ELISA
Synonyms
M13 Phage Titration; M13 Phage Titration ELISA Kit; M13 Phage Titration elisa kit
Specificity
This ELISA is specific for the measurement of natural and recombinant mouse periostin. It has been tested on mouse periostin isoforms 5 (should also detect mouse isoforms 1, 2 and 3 according to sequences). Mouse isoform 4 has not been tested.
Applicable Applications for M13 Phage Titration elisa kit
ELISA (EIA)
Samples
Cell Culture Supernatants, Serum And Plasma
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
10 pg/ml
Intra-assay Precision
Four samples of known concentrations of mouse periostin were assayed in replicates 8 times to test precision within an assay
Inter-assay Precision
Four samples of known concentrations of mouse periostin were assayed in 3 separate assays to test precision between assays.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for M13 Phage Titration elisa kit
Intended Uses: The Periostin mouse ELISA kit is to be used for the measurement of mouse periostin in cell culture supernatants, serum and plasma. This ELISA kit is for research use only.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of periostin in the samples!!Background/Introduction: Periostin, also termed osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein that shares a homology with the insect axon guidance protein fasciclin I (1). Periostin is one of four known mammalian genes that contain fasciclin domains with stabilin 1 and 2, as well as TGFbeta-Induced Gene-Human clone 3 (BIGH3) (2). Periostin protein is composed of a signal sequence, four-coiled fasciclin-like repeats, an aminoterminal cysteine-rich region (EMI domain), and heparin-binding domains present in the carboxyl tail. Periostin contains gamma-carboxyglutamate residues that are formed by vitamin K dependent carboxylation (3). These residues are essential for the binding of calcium. Periostin is thought to be involved in osteoblast recruitment, attachment and spreading. It is a component of the extracellular matrix The N-terminus part of periostin (up to exon 16) is conserved, while the C-terminal region (comprising exon 17-23) gives rise to different splice isoforms upon alternative splicing. The isoforms have a molecular weight range from 83 kDa to 93 kDa (4). Six different periostin splice isoforms have been reported, but only four of them were sequenced and annotated. Periostin is expressed during ontogenesis and down regulated in adult except in bones, in collagenrich fibrous connective tissues subjected to constant mechanical stress, such as periodontal ligament (PDL), heart valves, skin and tendons. Periostin expression is also observed in niches in direct contact with tissue-specific stem cells in mammary gland, bone, and intestine. Periostin has been found to be overexpressed in various type of human tumors including neuroblastoma, head and neck cancer, nasopharyngeal carcinoma, non-small cell lung carcinoma, breast cancer, colon cancer, pancratic ductal adenocarcinoma and ovarian cancer (5). Isoforms of periostin are over-expressed by stromal cells in several human ovary, breast, colon and brain tumors. Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. Periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour. Infiltrating tumour cells need to induce stromal Periostin expression in the secondary target organ to initiate colonization. Periostin is crucial for cancer stem cell maintenance (6). Periostin up-regulation in cancers usually correlates with aggressiveness and/or poor survival.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of periostin in the samples!!Background/Introduction: Periostin, also termed osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein that shares a homology with the insect axon guidance protein fasciclin I (1). Periostin is one of four known mammalian genes that contain fasciclin domains with stabilin 1 and 2, as well as TGFbeta-Induced Gene-Human clone 3 (BIGH3) (2). Periostin protein is composed of a signal sequence, four-coiled fasciclin-like repeats, an aminoterminal cysteine-rich region (EMI domain), and heparin-binding domains present in the carboxyl tail. Periostin contains gamma-carboxyglutamate residues that are formed by vitamin K dependent carboxylation (3). These residues are essential for the binding of calcium. Periostin is thought to be involved in osteoblast recruitment, attachment and spreading. It is a component of the extracellular matrix The N-terminus part of periostin (up to exon 16) is conserved, while the C-terminal region (comprising exon 17-23) gives rise to different splice isoforms upon alternative splicing. The isoforms have a molecular weight range from 83 kDa to 93 kDa (4). Six different periostin splice isoforms have been reported, but only four of them were sequenced and annotated. Periostin is expressed during ontogenesis and down regulated in adult except in bones, in collagenrich fibrous connective tissues subjected to constant mechanical stress, such as periodontal ligament (PDL), heart valves, skin and tendons. Periostin expression is also observed in niches in direct contact with tissue-specific stem cells in mammary gland, bone, and intestine. Periostin has been found to be overexpressed in various type of human tumors including neuroblastoma, head and neck cancer, nasopharyngeal carcinoma, non-small cell lung carcinoma, breast cancer, colon cancer, pancratic ductal adenocarcinoma and ovarian cancer (5). Isoforms of periostin are over-expressed by stromal cells in several human ovary, breast, colon and brain tumors. Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. Periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour. Infiltrating tumour cells need to induce stromal Periostin expression in the secondary target organ to initiate colonization. Periostin is crucial for cancer stem cell maintenance (6). Periostin up-regulation in cancers usually correlates with aggressiveness and/or poor survival.
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Product Notes
The M13 Phage Titration (Catalog #AAA539756) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's M13 Phage Titration can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the M13 Phage Titration for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "M13 Phage Titration, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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