Typical Testing Data/Standard Curve (for reference only)
Mouse Periostin/OSF-2 ELISA Kit
Periostin / OSF-2 (mouse) ELISA Kit
Reactivity
Mouse
Synonyms
Periostin/OSF-2; Periostin / OSF-2 (mouse) ELISA Kit; Osteoblast-specific Factor 2 (mouse) ELISA Kit; OSF-2 (mouse) ELISA Kit; Postn (mouse) ELISA Kit; Periostin/OSF-2 elisa kit
Reactivity
Mouse
Application Notes
Quantitative ELISA
Samples
Serum & plasma, Cell Culture Supernatants
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay Periostin.
The minimum detectable concentration of Irisin is 10 pg/ml.
This ELISA is specific for the measurement of natural and recombinant mouse periostin. It has been tested on mouse periostin isoform 5 (should also detect mouse isoforms 1, 2 and 3 according to sequences). Mouse isoform 4 has not been tested.
The minimum detectable concentration of Irisin is 10 pg/ml.
This ELISA is specific for the measurement of natural and recombinant mouse periostin. It has been tested on mouse periostin isoform 5 (should also detect mouse isoforms 1, 2 and 3 according to sequences). Mouse isoform 4 has not been tested.
Preparation and Storage
4 degree C
Shelf Life: 12 months after the day of manufacturing
Shelf Life: 12 months after the day of manufacturing
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Periostin/OSF-2 elisa kit
Product Information: Periostin/OSF-2 (mouse) ELISA Kit: Colorimetric Assay for Quantitative Determination of mouse Periostin in Serum, Plasma & Cell culture supernatants. Detection Limit: 10 pg/ml. 100 assays.
Background: MyBioSource's Periostin (mouse) ELISA kit is to be used for the in vitro quantitative determination of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of periostin in the samples. Detection limit: 10 pg/ml. Note: The Limit of detection was measured by adding three standard deviations to the mean value of 50 zero standard. Assay Range: 31.25 pg/ml - 2000 pg/ml. Linearity: Different samples containing mouse periostin were diluted several fold (1/4000 to 1/8000) and the measured recoveries ranged from 95% to 105%. Mouse periostin levels range in plasma or serum from 1 to >10 mug /ml. Recovery: When samples (serum or plasma) are spiked with known concentrations of mouse periostin, the recovery averages 96% (range from 89% to 115%).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- mouse
Application- Quantitative ELISA
Background: MyBioSource's Periostin (mouse) ELISA kit is to be used for the in vitro quantitative determination of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of periostin in the samples. Detection limit: 10 pg/ml. Note: The Limit of detection was measured by adding three standard deviations to the mean value of 50 zero standard. Assay Range: 31.25 pg/ml - 2000 pg/ml. Linearity: Different samples containing mouse periostin were diluted several fold (1/4000 to 1/8000) and the measured recoveries ranged from 95% to 105%. Mouse periostin levels range in plasma or serum from 1 to >10 mug /ml. Recovery: When samples (serum or plasma) are spiked with known concentrations of mouse periostin, the recovery averages 96% (range from 89% to 115%).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- mouse
Application- Quantitative ELISA
Product Categories/Family for Periostin/OSF-2 elisa kit
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Product Notes
The Mouse Periostin/OSF-2 (Catalog #AAA843369) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843369 ELISA Kit recognizes Mouse Periostin/OSF-2. Quantitative ELISA. Researchers should empirically determine the suitability of the Periostin/OSF-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Periostin/OSF-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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