Rabbit Thr306 CaM Kinase II Polyclonal Antibody | anti-CAMK2A/B antibody

Anti-Phospho-Thr306 CaM Kinase II

Cat. Num. AAA502225

• Reactivity: Bovine, Canine, Chicken, Human, Xenopus, Zebrafish
• Applications: Western Blot
• Purity: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Synonyms: Thr306 CaM Kinase II; Polyclonal Antibody; Anti-Phospho-Thr306 CaM Kinase II; CaM Kinase II (Thr306); anti-CAMK2A/B antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Chicken, Human, Xenopus, Zebrafish
• Clonality: Polyclonal
• Specificity: Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by lambda-phosphatase treatment. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands in native tissue, the antibody reacts in a phospho-specific manner.
• Purity/Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 425

• Applicable Applications for anti-CAMK2A/B antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen Species: Rat
• Immunogen Info: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 conjugated to KLH
• Biological Significance: Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). CaMKIIalpha autophosphorylation at Thr286 and Thr305/Thr306 has recently been shown to regulate kinase activity and modulate subcellular targeting and is critical for normal synaptic plasticity and learning and memory (Baucum et al., 2015).
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr306 found in rat brain CaM Kinase II.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot of rat brain lysate showing specific immunolabeling of the ~50k alpha- and the ~60k beta- CaM Kinase II phosphorylated at Thr306 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.)

NCBI and Uniprot Product Information

• NCBI GI #: 446672784
• NCBI GeneID: 20493238
• NCBI Accession #: WP_000750130.1
• NCBI GenBank Nucleotide #: WP_000750130.1
• UniProt Accession #: P11295; O06897; O30770
• Molecular Weight: ~50 kDa; ~60 kDa
• NCBI Official Full Name: MULTISPECIES: L-lysine N6-monooxygenase
• NCBI Official Symbol: D616_p153076
• NCBI Protein Information: aerobactin biosynthesis protein IucD
• UniProt Protein Name: L-lysine N6-monooxygenase
• UniProt Gene Name: iucD
• UniProt Synonym Gene Names: aerA
• UniProt Entry Name: IUCD_ECOLX

Uniprot Description

Flavoprotein monooxygenase required for N-hydroxylation of lysine. Involved in the biosynthesis of the siderophore aerobactin which is a chelator that mediates the high-affinity iron transport systems induced by the organism under iron-stressed conditions.