Rabbit Ser554 DENND3 Polyclonal Antibody | anti-DENND3 antibody

Anti-Phospho-Ser554 DENND3

Cat. Num. AAA502222

• Reactivity: Human, Mouse, Non-Human Primate, Rat, Sheep
• Applications: Western Blot
• Purity: Affinity purified rabbit polyclonal antibody
• Synonyms: Ser554 DENND3; Polyclonal Antibody; Anti-Phospho-Ser554 DENND3; DENND3 (Ser554); anti-DENND3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Non-Human Primate, Rat, Sheep
• Clonality: Polyclonal
• Specificity: Specific for the ~142kD DENND3 phosphorylated at Ser⁵⁵⁴. Immunolabeling is blocked by lambda-phosphate treatment
• Purity/Purification: Affinity purified rabbit polyclonal antibody
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini western blots
• Sequence Length: 1,278

• Applicable Applications for anti-DENND3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500
• Quality Control Tests: Western Blot performed on each lot
• Biological Significance: The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011). ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (McPherson and Xu, 2015).
• Preparation and Storage: Store at -20 degree C; stable for one year

Western Blot (WB)

(Western blot of HeLa lysate showing specific immunolabeling of the ~142kD DENND3 protein phosphorylated at Ser554 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.)

NCBI and Uniprot Product Information

• UniProt Accession #: D4A2H4
• Molecular Weight: ~142 kDa
• UniProt Protein Name: Protein Dennd3
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: Dennd3
• UniProt Entry Name: D4A2H4_RAT

Uniprot Description

DENND3: a guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Plays a role in protein transport from recycling endosomes to lysosomes regulating, for example, the degradation of the transferrin receptor. Four isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rab

Cellular Component: intracellular

Molecular Function: Rab guanyl-nucleotide exchange factor activity

Biological Process: cellular protein catabolic process; endosome to lysosome transport; positive regulation of GTPase activity