Rabbit Ser556 FANCI Polyclonal Antibody | anti-FANCI antibody

Anti-Phospho-Ser556 FANCI

Cat. Num. AAA502228

• Gene Names: FANCI; KIAA1794
• Reactivity: Bovine, Non-Human Primates, Goat, Sheep
• Applications: Western Blot
• Purity: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Synonyms: Ser556 FANCI; Polyclonal Antibody; Anti-Phospho-Ser556 FANCI; FANCI (Ser556); anti-FANCI antibody

• Host: Rabbit
• Reactivity: Bovine, Non-Human Primates, Goat, Sheep
• Clonality: Polyclonal
• Specificity: Specific for the ~150k FANCI protein phosphorylated at Ser556. Immunolabeling of FANCI is completely eliminated by lambda-phosphatase treatment.
• Purity/Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 1328

• Applicable Applications for anti-FANCI antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen Species: Human
• Immunogen Info: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 conjugated to KLH
• Biological Significance: Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser 556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser556 of human FANCI.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot of HeLa cell lysate showing specific immunolabeling of the ~150kd FANCI protein phosphorylated at Ser556 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.)

NCBI and Uniprot Product Information

• NCBI GI #: 164607124
• NCBI GeneID: 55215
• NCBI Accession #: NP_001106849.1
• NCBI GenBank Nucleotide #: NM_001113378.1
• UniProt Accession #: Q9NVI1; Q96JN1; Q96ST0; Q9BT96; A4ZVE4; A5YMH4; A6NJZ0
• Molecular Weight: ~150 kDa
• NCBI Official Full Name: Fanconi anemia group I protein isoform 1
• NCBI Official Synonym Full Names: Fanconi anemia complementation group I
• NCBI Official Symbol: FANCI
• NCBI Official Synonym Symbols: KIAA1794
• NCBI Protein Information: Fanconi anemia group I protein
• UniProt Protein Name: Fanconi anemia group I protein
• Protein Family: Fanconi anemia group I protein
• UniProt Gene Name: FANCI
• UniProt Synonym Gene Names: KIAA1794; Protein FACI
• UniProt Entry Name: FANCI_HUMAN

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group I. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FANCI: Plays an essential role in the repair of DNA double- strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single- stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage. Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI). A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cytoplasm; membrane; nucleoplasm

Molecular Function: DNA binding; protein binding

Biological Process: mitotic cell cycle G2/M transition DNA damage checkpoint; positive regulation of protein ubiquitination

Disease: Fanconi Anemia, Complementation Group I; Tracheoesophageal Fistula With Or Without Esophageal Atresia