Rabbit anti-Rat GluR1-Subunit (Ser845) Polyclonal Antibody | anti-GRIA1 antibody

Anti-Phospho-Ser845 GluR1

Cat. Num. AAA502063

• Gene Names: Gria1; GluA1; gluR-A
• Reactivity: Rat
• Applications: Western Blot, Dot Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: GluR1-Subunit (Ser845); Polyclonal Antibody; Anti-Phospho-Ser845 GluR1; anti-GRIA1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is s completely eliminated by treatment with lambda-Ptase.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 150 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 907

• Applicable Applications for anti-GRIA1 antibody: Western Blot (WB), Dot Blots (DB), Immunohistochemistry (IHC) has not yet been determined
• Application Notes: WB: 1:1000; IHC: 1:1000 (frozen sections); Quality Control Test: Western Blot performed on each lot.
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Human, mouse, non-human primates
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as arning and memory (Lee at al., 2003; Esteban at al., 2003).
• Preparation and Storage: For long term storage -80 degree C is recommended. Shorterm storage at -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 yr at -20 degree C.

Testing Data

Related Product Information for anti-GRIA1 antibody

Affinity purified rabbit polyclonal antibody

References

• Banke TG, Bowie D, Lee HK, Huganir RL, Schousboe A, Traynelis SF (2000) Control of GluR1 AMPA receptor function by cAMP-dependent protein kinase. J Neurosci 20:89-102.
• Esteban JA, Shi SH, Wilson C, Nuriya M, Huganir RL, Malinow R (2003) PKA phosphorylation of AMPA receptor subunits controls synaptic trafficking underlying plasticity. Nature Neurosci 6:136-143.
• Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108.
• Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560.
• Lee HK, Takamiya K, Han JS, Man HY, Kim CH, Rumbaugh G, Yu S, Ding L, He C, Petralia RS, Wenthold RJ, Gallagher M, Huganir RL (2003) Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory. Cell 112(5):631-643.

NCBI and Uniprot Product Information

• NCBI GI #: 29789269
• NCBI GeneID: 50592
• NCBI Accession #: NP_113796.1
• NCBI GenBank Nucleotide #: NM_031608.1
• UniProt Accession #: P19490
• Molecular Weight: 100
• NCBI Official Full Name: glutamate receptor 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 1
• NCBI Official Symbol: Gria1
• NCBI Official Synonym Symbols: GluA1; gluR-A
• NCBI Protein Information: glutamate receptor 1; GluR1; gluR-1; gluR-K1; glutamate receptor A; glutamate receptor subunit GluR1; AMPA-selective glutamate receptor 1; glutamate receptor, ionotropic, AMPA1 (alpha 1)
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: Gria1
• UniProt Synonym Gene Names: Glur1; GluR-1; GluA1
• UniProt Entry Name: GRIA1_RAT

NCBI Description

mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning [RGD, Feb 2006]

Uniprot Description

Function: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Ref.12 Ref.17

Subunit structure: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with HIP1, RASGRF2, SYNDIG1 and LRFN1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain).

Subcellular location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density

By similarity. Cell projection › dendrite

By similarity. Cell projection › dendritic spine

By similarity. Note: Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Ref.11 Ref.12 Ref.17

Tissue specificity: Detected in cerebellum (at protein level). Ref.11

Domain: The M4 transmembrane segment mediates tetramerization and is required for cell surface expression

By similarity.

Post-translational modification: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis

By similarity.

Polymorphism: Both variants Ser-710 and Thr-710 are phosphorylated at this position.

Miscellaneous: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. [View classification]