Typical Testing Data/Standard Curve (for reference only)
Human Prostaglandin G/H synthase 2 ELISA Kit | PTGS2 elisa kit
Human Prostaglandin G/H synthase 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Uniprot Description
COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.
Protein type: Oxidoreductase; EC 1.14.99.1; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 1q25.2-q25.3
Cellular Component: caveola; cytoplasm; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; neuron projection; nucleus; protein complex
Molecular Function: arachidonate 15-lipoxygenase activity; enzyme binding; heme binding; lipid binding; metal ion binding; peroxidase activity; prostaglandin-endoperoxide synthase activity; protein binding; protein homodimerization activity
Biological Process: angiogenesis; arachidonic acid metabolic process; bone mineralization; brown fat cell differentiation; cell motility; cyclooxygenase pathway; decidualization; embryo implantation; hair cycle; inflammatory response; learning; lipoxygenase pathway; memory; NAD metabolic process; negative regulation of calcium ion transport; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of smooth muscle contraction; negative regulation of synaptic transmission, dopaminergic; nicotinamide metabolic process; ovulation; positive regulation of apoptosis; positive regulation of fever; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of prostaglandin biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle contraction; positive regulation of synaptic plasticity; positive regulation of synaptic transmission, glutamatergic; positive regulation of vasoconstriction; prostaglandin biosynthetic process; prostaglandin metabolic process; regulation of blood pressure; regulation of inflammatory response; response to drug; response to estradiol stimulus; response to fructose stimulus; response to glucocorticoid stimulus; response to lipopolysaccharide; response to lithium ion; response to manganese ion; response to oxidative stress; response to vitamin D; sensory perception of pain; vitamin metabolic process; water-soluble vitamin metabolic process
Research Articles on PTGS2
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Product Notes
The Human PTGS2 ptgs2 (Catalog #AAA288671) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288671 ELISA Kit recognizes Human PTGS2. It is sometimes possible for the material contained within the vial of "Prostaglandin G/H synthase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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