Rabbit anti-Rat CaM Kinase II (Thr286) Polyclonal Antibody | anti-CAMK2A/B antibody

Anti-Phospho-Thr286 CaM Kinase II

Cat. Num. AAA502009

• Gene Names: Camk2a; PKCCD; PK2CDD
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: CaM Kinase II (Thr286); Polyclonal Antibody; Anti-Phospho-Thr286 CaM Kinase II; anti-CAMK2A/B antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the lambda-phosphatase treatment.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 478

• Applicable Applications for anti-CAMK2A/B antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Human, mouse and Xenopus
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-CAMK2A/B antibody

Affinity purified rabbit polyclonal antibody

References

• Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ (2002) Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning. Neuron 36:493-505
• Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR (2003) Translocation of CaM kinase II to synaptic sites in vivo. Nature Neurosci 6:217-218.
• Hudmon A, Schulman H (2002) Neuronal Ca2+/calmodulin-dependent protein kinase II: The role of structure and autoregulation in cellular function. Annu Rev Biochem 71:473-510.
• Kennedy MB (1998) Signal transduction molecules at the glutamatergic postsynaptic membrane. Brain Res Rev 26:243-257.
• Meng FJ, Guo J, Zhang QG, Song B, Zhang GY (2003) Autophosphorylated calcium/calmodulin-dependent protein kinase IIa (CaMKIIa) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats. Brain Res 967:161-169.

NCBI and Uniprot Product Information

• NCBI GI #: 6978593
• NCBI GeneID: 25400
• NCBI Accession #: NP_037052.1
• NCBI GenBank Nucleotide #: NM_012920.1
• UniProt Accession #: P11275
• Molecular Weight: 50/60
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: Camk2a
• NCBI Official Synonym Symbols: PKCCD; PK2CDD
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; alpha CaM kinase II; caMK-II subunit alpha; caM-kinase II alpha chain; caM kinase II subunit alpha; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• UniProt Gene Name: Camk2a
• UniProt Synonym Gene Names: CaM kinase II subunit alpha
• UniProt Entry Name: KCC2A_RAT

Uniprot Description

Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.

Subunit structure: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1. Ref.5 Ref.7 Ref.8 Ref.9

Subcellular location: Cell junction › synapse › presynaptic cell membrane. Cell junction › synapse. Note: Postsynaptic lipid rafts. Ref.9

Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Contains 1 protein kinase domain.