vSET recombinant protein
Recombinant vSET (A612L) protein
Synonyms
vSET; Recombinant vSET (A612L) protein; vSET recombinant protein
Host
E Coli
Form/Format
5ug of recombinant vSET (A612L) expressed in E Coli and supplied in a buffer of 30mM HEPES, pH7.4, 500mM NaCl, and 2.5% 1,2-Propanediol.
Protein Species
Viral
Tag
His-Tag
Notes
Recombinant vSET (A612L) is suitable for use in histone methyltransferase (HMT) assays. The preferred substrate sequence for vSET is #RKXA, where # represents an amino acid with small side chain and X represents any amino acid. The binding stoichiometry of H3 peptide to vSET dimer is 1:1. Assay conditions: Assay buffer: 20mM Tris, pH 8.0, 20mM KCl, 10mM MgCl2. We recommend a concentration of 1uM vSET (A612L), 0.1mM histone H3 peptide, and 1mM S-adenosyl methionine for each assay. The reaction is performed at ambient temperature. 1% trifluoroacetic acid can be added to stop the reaction.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Related Product Information for vSET recombinant protein
Short Description: Recombinant vSET is suitable for use in histone methyltransferase (HMT) assays. Recombinant vSET protein was expressed in E Coli and contains an N-terminal His-tag (MW = 13 kDa). vSET is a viral protein encoded by paramecium bursaria chlorella virus 1 (PBCV-1), and has explicit methyltransferase activity for host histone H3 lysine 27. It has been shown that vSET can exert its activity by mimicking the function of EZH proteins of the mammalian Polycomb Repressive Complex 2 (PRC2) that are the only histone methyltransferases (HMTs) known to methylate H3K27. In contrast to mammalian HMTs that are monomeric and require binding partners for optimal methyltransferase activity, vSET functions as a dimer. It has been shown that vSET alone is required for global methylation of H3K27, unlike mammalian EZH2 HMT that is only capable of global H3K27 methylation in the context of the Polycomb Repressive Complex. vSET catalyzes H3K27 dimethylation and, to a lesser extent, mono- and trimethylation. It exerts activity on both free and nucleosome-associated H3. Recombinant vSET can be used in histone methyltransferase (HMT) assays.
Background: vSET is a viral protein encoded by paramecium bursaria chlorella virus 1 (PBCV-1), and has explicit methyltransferase activity for host histone H3 lysine 27. It has been shown that vSET can exert its activity by mimicking the function of EZH proteins of the mammalian Polycomb Repressive Complex 2 (PRC2) that are the only histone methyltransferases (HMTs) known to methylate H3K27. In contrast to mammalian HMTs that are monomeric and require binding partners for optimal methyltransferase activity, vSET functions as a dimer. It has been shown that vSET alone is required for global methylation of H3K27, unlike mammalian EZH2 HMT that is only capable of global H3K27 methylation in the context of the Polycomb Repressive Complex. vSET catalyzes H3K27 dimethylation and, to a lesser extent, mono- and trimethylation. It exerts activity on both free and nucleosome-associated H3.
Background: vSET is a viral protein encoded by paramecium bursaria chlorella virus 1 (PBCV-1), and has explicit methyltransferase activity for host histone H3 lysine 27. It has been shown that vSET can exert its activity by mimicking the function of EZH proteins of the mammalian Polycomb Repressive Complex 2 (PRC2) that are the only histone methyltransferases (HMTs) known to methylate H3K27. In contrast to mammalian HMTs that are monomeric and require binding partners for optimal methyltransferase activity, vSET functions as a dimer. It has been shown that vSET alone is required for global methylation of H3K27, unlike mammalian EZH2 HMT that is only capable of global H3K27 methylation in the context of the Polycomb Repressive Complex. vSET catalyzes H3K27 dimethylation and, to a lesser extent, mono- and trimethylation. It exerts activity on both free and nucleosome-associated H3.
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Product Notes
The vSET (Catalog #AAA388120) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "vSET, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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