SDS-Page
(Recombinant PRC2 EZH2 (Y641F) complex protein gel. PRC2 EZH2(Y641F) complex was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW of components: EZH2 (Y641F): 87 kDa. SUZ12: 83 kDa. EED: 50.2 kDa. RbAp46: 47.8 kDa. RbAp48: 47.7 kDa. Purity: 85%)
PRC2 EZH2 Recombinant Protein | PRC2EZH2 recombinant protein
Recombinant PRC2 EZH2 (Y641F) complex
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
PRC2 EZH2; Recombinant PRC2 EZH2 (Y641F) complex; PRC2EZH2 recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Recombinant PRC2 EZH2 (Y641F) complex is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
PRC2 EZH2(Y641F) complex is suitable for use in the study of enzyme kinetics, inhibitor screening and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PRC2 EZH2 (Y641F) complex protein gel. PRC2 EZH2(Y641F) complex was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW of components: EZH2 (Y641F): 87 kDa. SUZ12: 83 kDa. EED: 50.2 kDa. RbAp46: 47.8 kDa. RbAp48: 47.7 kDa. Purity: 85%)
SDS-Page
(Recombinant PRC2 EZH2 (Y641F) complex protein gel. PRC2 EZH2(Y641F) complex was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW of components: EZH2 (Y641F): 87 kDa. SUZ12: 83 kDa. EED: 50.2 kDa. RbAp46: 47.8 kDa. RbAp48: 47.7 kDa. Purity: 85%)
Testing Data
(HTRF Assay for Recombinant PRC2 EZH2(Y641F) complex activity. 1 ?M histone H3K27me2 (21-44aa.) peptide was incubated with different concentrations of Recombinant PRC2 EZH2 (Y641F) Complex in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours at room temperature. Then 10 ?l anti-H3K27me3 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Testing Data
(HTRF Assay for Recombinant PRC2 EZH2(Y641F) complex activity. 1 ?M histone H3K27me2 (21-44aa.) peptide was incubated with different concentrations of Recombinant PRC2 EZH2 (Y641F) Complex in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours at room temperature. Then 10 ?l anti-H3K27me3 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Testing Data
(HTRF Assay for Recombinant PRC2 EZH2(Y641F) complex activity. 1 ?M histone H3K27me2 (21-44aa.) peptide was incubated with different concentrations of Recombinant PRC2 EZH2 (Y641F) Complex in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours at room temperature. Then 10 ?l anti-H3K27me3 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Testing Data
(HTRF Assay for Recombinant PRC2 EZH2(Y641F) complex activity. 1 ?M histone H3K27me2 (21-44aa.) peptide was incubated with different concentrations of Recombinant PRC2 EZH2 (Y641F) Complex in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours at room temperature. Then 10 ?l anti-H3K27me3 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Related Product Information for PRC2EZH2 recombinant protein
Short Description: Recombinant PRC2 EZH2 (Y641F) complex that includes full-length EZH2 with a Y641F mutation complexed with full-length SUZ12, EED and RbAp46/48 (accession numbers NP_001190176.1, NP_056170, NP_003788.2, NP_002884.1, and NP_005601.1, respectively) was expressed in Sf9 and contains an N-terminal DYKDDDDK tag at the N-terminus of EZH2. The molecular weights of expressed EZH2 (Y641F), SUZ12, EED and RbAp46/48 are 87 kDa, 83 kDa, 50.2 kDa, 47.8 kDa and 47.7 kDa, respectively. PRC2 (Polycomb Repressive Complex 2) is one of the two classes Polycomb-group, or PcG proteins (the other being PRC1) that are important epigenetic determinants of stem cell identity. They play an important role in long-term epigenetic silencing of genes during cell fate determination and differentiation. PRC2 functions as a repressor of chromatin. PRC2 is required to target recruitment to specific DNA sequences (termed Polycomb Response Elements or PREs) of genomic regions to be silenced. Once associated with chromatin, the PRC2 subunit EZH2 has histone methyltransferase activity that catalyzes the trimethylation of histone H3 at Lys27. H3K27me3 is well established as a hallmark of regions of repressed chromatin. Trimethylation of Lys27 leads to the recruitment of PRC1 through the binding of H3K27me3 by chromodomain-containing proteins in PRC1. PRC1 is responsible for long-term gene silencing after cellular differentiation. Recombinant PRC2 EZH2 (Y641F) complex is a mutant version of our wild-type PRC2 complex that contains a Tyr-to-Phe mutation at tyrosine 641 of the SET domain of EZH2. Somatic mutations of tyrosine 641, including Y641F, Y641N, Y641C, Y641S and Y641H, have been identified in patients with non-Hodgkin lymphoma and have been shown to alter substrate specificity and catalytic activity of EZH2 for histone H3 lysine 27 (H3K27) methylation states resulting in increased H3K27 trimethylation. PRC2 EZH2 (Y641F) complex can be used to study enzyme kinetics and perform inhibitor screening.
Background: PRC2 (Polycomb Repressive Complex 2) is one of the two classes Polycomb-group, or PcG proteins (the other being PRC1) that are important epigenetic determinants of stem cell identity. They play an important role in long-term epigenetic silencing of genes during cell fate determination and differentiation. PRC2 functions as a repressor of chromatin. PRC2 is required to target recruitment to specific DNA sequences (termed Polycomb Response Elements or PREs) of genomic regions to be silenced. Once associated with chromatin, the PRC2 subunit EZH2 has histone methyltransferase activity that catalyzes the trimethylation of histone H3 at Lys27. H3K27me3 is well established as a hallmark of regions of repressed chromatin. Trimethylation of Lys27 leads to the recruitment of PRC1 through the binding of H3K27me3 by chromodomain-containing proteins in PRC1. PRC1 is responsible for long-term gene silencing after cellular differentiation. Recombinant PRC2 EZH2(Y641F) Complex is a mutant version of our wild-type PRC2 Complex that contains a Tyr-to-Phe mutation at tyrosine 641 of the SET domain of EZH2. Somatic mutations of tyrosine 641, including Y641F, Y641N, Y641C, Y641S and Y641H, have been identified in patients with non-Hodgkin lymphoma and have been shown to alter substrate specificity and catalytic activity of EZH2 for histone H3 lysine 27 (H3K27) methylation states resulting in increased H3K27 trimethylation.
Background: PRC2 (Polycomb Repressive Complex 2) is one of the two classes Polycomb-group, or PcG proteins (the other being PRC1) that are important epigenetic determinants of stem cell identity. They play an important role in long-term epigenetic silencing of genes during cell fate determination and differentiation. PRC2 functions as a repressor of chromatin. PRC2 is required to target recruitment to specific DNA sequences (termed Polycomb Response Elements or PREs) of genomic regions to be silenced. Once associated with chromatin, the PRC2 subunit EZH2 has histone methyltransferase activity that catalyzes the trimethylation of histone H3 at Lys27. H3K27me3 is well established as a hallmark of regions of repressed chromatin. Trimethylation of Lys27 leads to the recruitment of PRC1 through the binding of H3K27me3 by chromodomain-containing proteins in PRC1. PRC1 is responsible for long-term gene silencing after cellular differentiation. Recombinant PRC2 EZH2(Y641F) Complex is a mutant version of our wild-type PRC2 Complex that contains a Tyr-to-Phe mutation at tyrosine 641 of the SET domain of EZH2. Somatic mutations of tyrosine 641, including Y641F, Y641N, Y641C, Y641S and Y641H, have been identified in patients with non-Hodgkin lymphoma and have been shown to alter substrate specificity and catalytic activity of EZH2 for histone H3 lysine 27 (H3K27) methylation states resulting in increased H3K27 trimethylation.
Product Categories/Family for PRC2EZH2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
746
NCBI Official Full Name
histone-lysine N-methyltransferase EZH2 isoform c
NCBI Official Synonym Full Names
enhancer of zeste homolog 2 (Drosophila)
NCBI Official Symbol
EZH2
NCBI Official Synonym Symbols
WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
NCBI Protein Information
histone-lysine N-methyltransferase EZH2; lysine N-methyltransferase 6
UniProt Protein Name
Histone-lysine N-methyltransferase EZH2
UniProt Gene Name
EZH2
UniProt Synonym Gene Names
KMT6
UniProt Entry Name
EZH2_HUMAN
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Product Notes
The PRC2EZH2 ezh2 (Catalog #AAA388108) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRC2 EZH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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