SDS-Page
(Recombinant SUV39H1 (82-412) protein 10% SDS-PAGE Coomassie staining MW: 64.6 kDa Purity: > 78% )
SUV39H1 recombinant protein
Recombinant SUV39H1 (82-412) protein
Gene Names
SUV39H1; MG44; KMT1A; SUV39H; H3-K9-HMTase 1
Synonyms
SUV39H1; Recombinant SUV39H1 (82-412) protein; MG44; KMT1A; SUV39H; H3-K9-HMTase 1; SUV39H1 recombinant protein
Host
E Coli
Form/Format
Recombinant SUV39H1 (82-412) protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 10% glycerol, and 0.5mM TCEP.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant SUV39H1 (82-412) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SUV39H1 (82-412) protein 10% SDS-PAGE Coomassie staining MW: 64.6 kDa Purity: > 78% )
SDS-Page
(Recombinant SUV39H1 (82-412) protein 10% SDS-PAGE Coomassie staining MW: 64.6 kDa Purity: > 78% )
Testing Data
(HTRF assay for SUV39H1 (82-412) activity 3 uM H3K9me1 (1-21 aa) peptide was incubated with different concentrations of SUV39H1 (82-412) protein in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Testing Data
(HTRF assay for SUV39H1 (82-412) activity 3 uM H3K9me1 (1-21 aa) peptide was incubated with different concentrations of SUV39H1 (82-412) protein in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Testing Data
(HTRF assay for SUV39H1 (82-412) activity 3 uM H3K9me1 (1-21 aa) peptide was incubated with different concentrations of SUV39H1 (82-412) protein in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Testing Data
(HTRF assay for SUV39H1 (82-412) activity 3 uM H3K9me1 (1-21 aa) peptide was incubated with different concentrations of SUV39H1 (82-412) protein in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Related Product Information for SUV39H1 recombinant protein
Short Description: Recombinant SUV39H1 (82-412) protein corresponding to amino acids 82-412 of SUV39H1 protein (accession number NP_003164.1) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 64.6 kDa. Recombinant SUV39H1 (82-412) protein contains the SET domain of SUV39H1 that is responsible for methyltransferase activity. It is suitable for use in histone methyltransferase assays as well as in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1, also known as KMT1A) is a histone methyltransferase that specifically trimethylates Lys-9 of histone H3, which results in transcriptional gene silencing. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. SUV39H1 and SUV39H2 can regulate the telomere length in mammalian cell. Histone H3 Lys-9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. H3K9me3 plays an important role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3K9me3 is also required to direct DNA methylation at pericentric repeats.
Background: SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1, also known as KMT1A) is a histone methyltransferase that specifically trimethylates Lys-9 of histone H3, which results in transcriptional gene silencing. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. SUV39H1 and SUV39H2 can regulate the telomere length in mammalian cell. Histone H3 Lys-9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. H3K9me3 plays an important role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3K9me3 is also required to direct DNA methylation at pericentric repeats.
Product Categories/Family for SUV39H1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
412
NCBI Official Full Name
histone-lysine N-methyltransferase SUV39H1 isoform 2
NCBI Official Synonym Full Names
suppressor of variegation 3-9 homolog 1 (Drosophila)
NCBI Official Symbol
SUV39H1
NCBI Official Synonym Symbols
MG44; KMT1A; SUV39H; H3-K9-HMTase 1
NCBI Protein Information
histone-lysine N-methyltransferase SUV39H1; Su(var)3-9 homolog 1; lysine N-methyltransferase 1A; histone H3-K9 methyltransferase 1; position-effect variegation 3-9 homolog; histone-lysine N-methyltransferase, H3 lysine-9 specific 1
UniProt Protein Name
Histone-lysine N-methyltransferase SUV39H1
Protein Family
UniProt Gene Name
SUV39H1
UniProt Synonym Gene Names
KMT1A; SUV39H; H3-K9-HMTase 1
UniProt Entry Name
SUV91_HUMAN
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Product Notes
The SUV39H1 suv39h1 (Catalog #AAA389080) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SUV39H1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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