SDS-Page
(Recombinant (SETD2 (1418-1714) protein gel. SETD2 (1418-1714 protein was run on an SDS-PAGE gel and stained with Coomassie blue.)
SETD2 recombinant protein
Recombinant SETD2 (1418-1714) protein
Synonyms
SETD2; Recombinant SETD2 (1418-1714) protein; HYPB; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP; SETD2 recombinant protein
Host
E Coli
Form/Format
50ug recombinant SETD2 (1418-1714) expressed in E Coli and supplied in a buffer of 40mM Tris-HCl, pH8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 20% glycerol and 3mM DTT.
Protein Species
Human
Tag
GST-Tag
Protein Details
Amino acids 1418-1714 of SETD2 (accession number NM_014159) were expressed in E Coli with an N-terminal GST-Tag (MW = 61kDa).
Notes
Recombinant SETD2 (1418-1714) is suitable for use in histone methyltransferase assays. A good starting point is 50 to 500ng of enzyme per assay. Assay conditions: Prepare a 50ul reaction mix (50mM TRIS pH 8.8, 5mM MgCl2, 4mM DTT, 20uM S-adenosylmethionine, and varying amounts of SETD2 (1418-1714)) and add to substrate-coated wells. Incubate at room temperature for 1 hr. Add anti-methyl histone H3K36 antibody and incubate 1hr. Next, incubate 1h with the HRP-labeled secondary antibody followed by chemiluminescent detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant (SETD2 (1418-1714) protein gel. SETD2 (1418-1714 protein was run on an SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(Recombinant (SETD2 (1418-1714) protein gel. SETD2 (1418-1714 protein was run on an SDS-PAGE gel and stained with Coomassie blue.)
Testing Data
(SETD2 (1418-1714) activity assay. Recombinant SETD2 (1418-1714) activity measured using a chemiluminescent methyltransferase assay.)
Testing Data
(SETD2 (1418-1714) activity assay. Recombinant SETD2 (1418-1714) activity measured using a chemiluminescent methyltransferase assay.)
Related Product Information for SETD2 recombinant protein
Short Description: Set2 is a histone methyltransferase that methylates histone H3 at lysine 36. Amino acids 1418-1714 of Set2 (accession number NM_014159) were expressed in E Coli with an N-terminal GST-Tag (MW = 61 kDa). Recombinant Set2 can be used in histone methyltransferase (HMT) assays.
Background: SETD2 (SET Domain Containing 2) is a Histone methyltransferase that specifically trimethylates Lys-36 of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. SETD2 plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. SETD2 acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
Background: SETD2 (SET Domain Containing 2) is a Histone methyltransferase that specifically trimethylates Lys-36 of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. SETD2 plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. SETD2 acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
Product Categories/Family for SETD2 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61kDa
Protein Family
Similar Products
Product Notes
The SETD2 (Catalog #AAA388084) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SETD2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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