SDS-Page
(EZH2 protein gel. EZH2 run on an SDS-PAGE gel and stained with Coomassie blue. Arrows indicate recombinant proteins as labeled.)
EZH2 recombinant protein
Recombinant EZH2 protein complex
Synonyms
EZH2; Recombinant EZH2 protein complex; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A; EZH2 recombinant protein
Host
Baculovirus
Form/Format
25ug recombinant EZH2 protein complex (contains EZH2, EED and Suz12 proteins) supplied in PBS (10mM Na2HPO4/KH2PO4), pH7.4, 2.7mM kCl, and 138mM NaCl.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant EZH2 protein complex is suitable for use in histone methyltransferase assays. Specific Activity: 0.08 pmol/min/ug. Assay conditions: Prepare a 50ul reaction mix (20mM phosphate pH 7.4, 0.05% Tween-20, 40uM S-adenosylmethionine, and 250-2000ng enzyme complex) and add to substrate-coated wells with the substrate on a Thermo Neutravidin black plate. Incubate for 1 hr. Add anti-methyl histone H3K27 antibody and incubate 1hr. Next, incubate 1h with the HRP-labeled secondary antibody followed by chemiluminescent detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(EZH2 protein gel. EZH2 run on an SDS-PAGE gel and stained with Coomassie blue. Arrows indicate recombinant proteins as labeled.)
SDS-Page
(EZH2 protein gel. EZH2 run on an SDS-PAGE gel and stained with Coomassie blue. Arrows indicate recombinant proteins as labeled.)
Testing Data
(EZH2 complex activity assay. Recombinant EZH2 activity measured using a fluorescent histone H3 lysine 27 methyltransferase assay.)
Testing Data
(EZH2 complex activity assay. Recombinant EZH2 activity measured using a fluorescent histone H3 lysine 27 methyltransferase assay.)
Related Product Information for EZH2 recombinant protein
Short Description: The EZH2 protein complex contains EZH2, EED and Suz12 which are the minimal components required for histone methyltransferase activity. EZH2 is specific for H3 lysine 27, a mark of repressed regions of chromatin. Recombinant human EZH2 (accession number NM_004456, amino acids 2-end with an N-terminal His-tag, molecular weight 86 kDa) was coexpressed in a baculovirus expression system along with human EED (accession number NM_003797, amino acids 2-end with an N-terminal DYKDDDDK tag, molecular weight 51 kDa) and human Suz12 (accession number NM_015355, amino acids 2-end with an N-terminal His-tag, molecular weight 87 kDa). EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2-(Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the >mono-, di- and trimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers. Recombinant EZH2 protein complex can be used in histone methyltransferase (HMT) assays.
Background: EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2-(Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the mono-, di- and trimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers.
Background: EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2-(Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the mono-, di- and trimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers.
Product Categories/Family for EZH2 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Protein Family
Similar Products
Product Notes
The EZH2 (Catalog #AAA388082) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EZH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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