SMYD3 recombinant protein

Recombinant SMYD3 protein

Cat. Num. AAA388125

• Gene Names: SMYD3; KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: SMYD3; Recombinant SMYD3 protein; KMT3E; ZMYND; ZNFN3A1; bA74P14.1; SMYD3 recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Recombinant SMYD3 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant SMYD3 protein gel. SMYD3 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 50.7 kDa Purity: > 90%)

Related Product Information for SMYD3 recombinant protein

Short Description: Recombinant SMYD3 (accession number NP_001161212.1) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 50.7 kDa. SET and MYND domain-containing protein 3 (SMYD3) is a member of the Smyd family of histone methyltransferases that specifically methylates lysine 4 of histone H3 (H3K4), inducing di- and trimethylation, but not monomethylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It has also been shown to bind DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. SMYD3 expression has been shown to be upregulated in certain cancers. Recombinant SMYD3 can be used to study enzyme kinetics and perform inhibitor screening assays.

Background: SET and MYND domain-containing protein 3 (SMYD3) is a member of the SMYD family of histone methyltransferases that specifically methylates lysine 4 of histone H3 (H3K4), inducing di- and tri-methylation, but not monomethylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It has also been shown to bind DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. SMYD3 expression has been shown to be upregulated in certain cancers.

Product Categories/Family for SMYD3 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT)

NCBI and Uniprot Product Information

• NCBI GI #: 267844824
• NCBI GeneID: 64754
• NCBI Accession #: NP_001161212.1
• NCBI GenBank Nucleotide #: NM_001167740.1
• UniProt Accession #: Q9H7B4; Q86TL8; Q8N5Z6; Q96AI5; A8K0P0; B1AN38
• Molecular Weight: 42,625 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SMYD3 isoform 1
• NCBI Official Synonym Full Names: SET and MYND domain containing 3
• NCBI Official Symbol: SMYD3
• NCBI Official Synonym Symbols: KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
• NCBI Protein Information: histone-lysine N-methyltransferase SMYD3
• UniProt Protein Name: Histone-lysine N-methyltransferase SMYD3
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SMYD3
• UniProt Synonym Gene Names: ZMYND1; ZNFN3A1
• UniProt Entry Name: SMYD3_HUMAN

NCBI Description

This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

SMYD3: Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: metal ion binding; histone-lysine N-methyltransferase activity

Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; establishment of protein localization; myotube cell development; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation