SDS-Page
(Recombinant SUV39H2 protein 10% SDS-PAGE Coomassie staining MW: 66.8 kDa Purity: > 55%)
SUV39H2 recombinant protein
Recombinant SUV39H2 protein
Gene Names
SUV39H2; KMT1B
Synonyms
SUV39H2; Recombinant SUV39H2 protein; KMT1B; SUV39H2 recombinant protein
Host
E Coli
Form/Format
Recombinant SUV39H2 protein is supplied at a concentration of 0.5ug/ul in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant SUV39H2 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 3uM H3K9me1 (1-21 aa) peptide was incubated with different concentrations of SUV39H1 (82-412) protein in a 10ul reaction system containing 50mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP and 50uM SAM for 1 hour, then 10ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SUV39H2 protein 10% SDS-PAGE Coomassie staining MW: 66.8 kDa Purity: > 55%)
SDS-Page
(Recombinant SUV39H2 protein 10% SDS-PAGE Coomassie staining MW: 66.8 kDa Purity: > 55%)
Testing Data
(HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
Testing Data
(HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
Testing Data
(HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
Testing Data
(HTRF assay for SUV39H2 activity 3 uM H3 (1-21) peptide was incubated with SUV39H2 in reaction buffer for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction products.)
Related Product Information for SUV39H2 recombinant protein
Short Description: Recombinant SUV39H2 was expressed in E Coli as the full-length protein (accession number NP_078946.1) with an N-terminal GST-Tag. The molecular weight of SUV39H2 is 66.8 kDa. It is suitable for use in histone methyltransferase (HMT) assays.
Background: SUV39H2 (Suppressor Of Variegation 3-9 Homolog 2, also known as KMT1B) is a histone methyltransferase that specifically trimethylates Lys-9 of histone H3. SUV39H2 prefers H3K9me0 as a substrate over H3K9me1 and H3K9me2. SUV39H2 can transfer up to three methyl groups to lysine 9 of histone H3 but the last methylation reaction is much slower than the first two steps. SUV39H1 and SUV39H2 can regulate the telomere length in mammalian cell. SuUV39H2 transcripts remain specifically expressed in adult testes. Histone H3 Lys-9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. H3K9me3 plays an important role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3K9me3 is also required to direct DNA methylation at pericentric repeats.
Background: SUV39H2 (Suppressor Of Variegation 3-9 Homolog 2, also known as KMT1B) is a histone methyltransferase that specifically trimethylates Lys-9 of histone H3. SUV39H2 prefers H3K9me0 as a substrate over H3K9me1 and H3K9me2. SUV39H2 can transfer up to three methyl groups to lysine 9 of histone H3 but the last methylation reaction is much slower than the first two steps. SUV39H1 and SUV39H2 can regulate the telomere length in mammalian cell. SuUV39H2 transcripts remain specifically expressed in adult testes. Histone H3 Lys-9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. H3K9me3 plays an important role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3K9me3 is also required to direct DNA methylation at pericentric repeats.
Product Categories/Family for SUV39H2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,350 Da
NCBI Official Full Name
histone-lysine N-methyltransferase SUV39H2 isoform 2
NCBI Official Synonym Full Names
suppressor of variegation 3-9 homolog 2
NCBI Official Symbol
SUV39H2
NCBI Official Synonym Symbols
KMT1B
NCBI Protein Information
histone-lysine N-methyltransferase SUV39H2
Protein Family
Research Articles on SUV39H2
Similar Products
Product Notes
The SUV39H2 (Catalog #AAA389074) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SUV39H2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
