Western Blot (WB)
(Western Blot: Sample: Rat Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat HSF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Heat Shock Transcription Factor 2 (HSF2) Polyclonal Antibody | anti-HSF2 antibody
Polyclonal Antibody to Heat Shock Transcription Factor 2 (HSF2)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Heat Shock Transcription Factor 2 (HSF2); Polyclonal Antibody; Polyclonal Antibody to Heat Shock Transcription Factor 2 (HSF2); anti-HSF2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HSF2. It has been selected for its ability to recognize HSF2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS FVRQLNMYGF RKVVHIESGI VKQERDGPVE FQHPHFKQGQ DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS FVRQLNMYGF RKVVHIESGI VKQERDGPVE FQHPHFKQGQ DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ
Sequence Length
536
Applicable Applications for anti-HSF2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant HSF2 (Met1~Leu200) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042727
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat HSF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat HSF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,744 Da
NCBI Official Full Name
heat shock factor protein 2
NCBI Official Synonym Full Names
heat shock transcription factor 2
NCBI Official Symbol
Hsf2
NCBI Protein Information
heat shock factor protein 2
UniProt Protein Name
Heat shock factor 2
Protein Family
UniProt Gene Name
Hsf2
NCBI Description
DNA binding protein; involved in the transcriptional regulation of heat shock protein (hsp) genes [RGD, Feb 2006]
Uniprot Description
HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 20q12
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: DNA binding; protein homodimerization activity; sequence-specific DNA binding
Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; spermatogenesis; transcription from RNA polymerase II promoter
Research Articles on HSF2
Similar Products
Product Notes
The HSF2 hsf2 (Catalog #AAA2015315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock Transcription Factor 2 (HSF2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Transcription Factor 2 (HSF2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSF2 hsf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS FVRQLNMYGF RKVVHIESGI VKQERDGPVE FQHPHFKQGQ DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ. It is sometimes possible for the material contained within the vial of "Heat Shock Transcription Factor 2 (HSF2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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