Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat AATF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Apoptosis Antagonizing Transcription Factor (AATF) Polyclonal Antibody | anti-AATF antibody
Polyclonal Antibody to Apoptosis Antagonizing Transcription Factor (AATF)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SALK NSHKALKALL RSLVDLQEEL LFQYPDTRYL VKGTKPNAES EEISSEDDEL VGEKKKQRKA PPKRKLEMED YPSFMAKRSA DFTVYRNRTL QKWHDKTKLA SGKLGKGFGA FERSILTQID HILMDKERLL RRTQTKRSAY RVLGKPEPVP EPVAETLPGE PESLPQVPAN AHLKDLDEEI FDDDDFYHQL LRELIERKTS SLDPNDQVAM GRQWLAIQKL RSKIRKKVDR KASKGRKLRF HVLSKLLSFM
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat AATF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat AATF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI Description
an apoptosis antagonizing nuclear phosphoprotein transcription factor [RGD, Feb 2006]
Uniprot Description
AATF: May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. Belongs to the AATF family.
Protein type: Nucleolus; RNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 10q26
Cellular Component: cytoplasm; Golgi apparatus; nucleolus; nucleus
Molecular Function: DNA binding transcription factor activity; leucine zipper domain binding; protein binding; protein domain specific binding; RNA binding; tau protein binding
Biological Process: apoptosis; cell adhesion; cellular response to DNA damage stimulus; embryonic cleavage; negative regulation of amyloid precursor protein biosynthetic process; negative regulation of apoptosis; negative regulation of superoxide release; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of mitotic cell cycle; regulation of transcription from RNA polymerase II promoter; ribosome biogenesis
Research Articles on AATF
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Product Notes
The AATF aatf (Catalog #AAA2014656) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apoptosis Antagonizing Transcription Factor (AATF) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Apoptosis Antagonizing Transcription Factor (AATF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the AATF aatf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SALK NSHKALKALL RSLVDLQEEL LFQYPDTRYL VKGTKPNAES EEISSEDDEL VGEKKKQRKA PPKRKLEMED YPSFMAKRSA DFTVYRNRTL QKWHDKTKLA SGKLGKGFGA FERSILTQID HILMDKERLL RRTQTKRSAY RVLGKPEPVP EPVAETLPGE PESLPQVPAN AHLKDLDEEI FDDDDFYHQL LRELIERKTS SLDPNDQVAM GRQWLAIQKL RSKIRKKVDR KASKGRKLRF HVLSKLLSFM. It is sometimes possible for the material contained within the vial of "Apoptosis Antagonizing Transcription Factor (AATF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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