Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human K562 Cells.)
Rabbit anti-Human Heat Shock Transcription Factor 1 (HSF1) Polyclonal Antibody | anti-HSF1 antibody
Polyclonal Antibody to Heat Shock Transcription Factor 1 (HSF1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG QFAKEVLPKY FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ HPCFLRGQEQ LLENIKRKVT SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK QECMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN RILGVKRKIP LMLNDSGSAH SMPKYSRQFS LEHVHGSGPY SAPSPAYSSS SLYAPDAVAS SGPIISDITE
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human K562 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human K562 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HSF1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HSF1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]
Uniprot Description
HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: centrosome; condensed chromosome kinetochore; cytoplasm; cytosol; euchromatin; heterochromatin; kinetochore; nucleoplasm; nucleus; perinuclear region of cytoplasm; PML body; pronucleus; ribonucleoprotein complex
Molecular Function: chromatin DNA binding; DNA binding; DNA binding transcription factor activity; heat shock protein binding; Hsp90 protein binding; identical protein binding; promoter-specific chromatin binding; protein binding; protein heterodimerization activity; protein kinase binding; protein self-association; sequence-specific DNA binding; sequence-specific single stranded DNA binding; translation elongation factor binding
Biological Process: cellular protein complex assembly; cellular response to angiotensin; cellular response to cadmium ion; cellular response to copper ion; cellular response to estradiol stimulus; cellular response to gamma radiation; cellular response to heat; cellular response to hydrogen peroxide; cellular response to L-glutamine; cellular response to nitroglycerin; cellular response to sodium arsenite; cellular response to unfolded protein; defense response; DNA repair; embryonic placenta development; embryonic process involved in female pregnancy; female meiotic nuclear division; MAPK cascade; mRNA processing; mRNA transcription; mRNA transport; negative regulation of cardiac muscle cell apoptotic process; negative regulation of cell proliferation; negative regulation of double-strand break repair via nonhomologous end joining; negative regulation of inclusion body assembly; negative regulation of neuron death; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; positive regulation of cell proliferation; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of inclusion body assembly; positive regulation of microtubule binding; positive regulation of mitotic cell cycle; positive regulation of mRNA polyadenylation; positive regulation of multicellular organism growth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of STAT protein; protein homooligomerization; regulation of cellular response to heat; regulation of protein heterodimerization activity; response to activity; response to hypobaric hypoxia; response to nutrient; response to psychosocial stress; response to testosterone stimulus; spermatogenesis
Research Articles on HSF1
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Product Notes
The HSF1 hsf1 (Catalog #AAA2014026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock Transcription Factor 1 (HSF1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Transcription Factor 1 (HSF1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSF1 hsf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG QFAKEVLPKY FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ HPCFLRGQEQ LLENIKRKVT SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK QECMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN RILGVKRKIP LMLNDSGSAH SMPKYSRQFS LEHVHGSGPY SAPSPAYSSS SLYAPDAVAS SGPIISDITE. It is sometimes possible for the material contained within the vial of "Heat Shock Transcription Factor 1 (HSF1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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