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Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse STAT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Signal Transducer And Activator Of Transcription 2 (STAT2) Polyclonal Antibody | anti-STAT2 antibody

Polyclonal Antibody to Signal Transducer And Activator Of Transcription 2 (STAT2)

Gene Names
Stat2; AW496480; 1600010G07Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Signal Transducer And Activator Of Transcription 2 (STAT2); Polyclonal Antibody; Polyclonal Antibody to Signal Transducer And Activator Of Transcription 2 (STAT2); anti-STAT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against STAT2. It has been selected for its ability to recognize STAT2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPL RFLYPRIPRD EAFGCYYQEK VNLEEQEEYL KHKLIVISNR QVDELQQPLE LKQDSESLEV NAELLLAHDQ ELPLMMQTGL VLGTELKVDP ILSTAPQVLL EPAPQVLLEP APQVLLEPAP QVLLEPAPQV LLEPAPQVLL EPAPQVLLEP APQVQLEPAP QVLLELAPQV LLEPAPQVLL ELAPQVQLEP AHLLQQPSES DLPEDLQQIS VEDLKKLSNP STEYITTNEN PMLAGESSGD ETSIPYHSHF
Sequence Length
857
Applicable Applications for anti-STAT2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant STAT2 (Asn658~Ala912) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2059224
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse STAT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse STAT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Uterus Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Uterus Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
105,417 Da
NCBI Official Full Name
Signal transducer and activator of transcription 2
NCBI Official Synonym Full Names
signal transducer and activator of transcription 2
NCBI Official Symbol
Stat2
NCBI Official Synonym Symbols
AW496480; 1600010G07Rik
NCBI Protein Information
signal transducer and activator of transcription 2
UniProt Protein Name
Signal transducer and activator of transcription 2
UniProt Gene Name
Stat2

Uniprot Description

STAT2: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Binds to the IFN-stimulated response element (ISRE) and to the Gas element. This multiprotein transcription factor is termed ISGF3. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 10 D3|10 76.5 cM

Cellular Component: cytoplasm; cytosol; nucleus; plasma membrane

Molecular Function: DNA binding; DNA binding transcription factor activity; identical protein binding; protein binding; signal transducer activity; ubiquitin-like protein ligase binding

Biological Process: defense response to virus; regulation of mitochondrial fission; regulation of protein amino acid phosphorylation; regulation of transcription, DNA-templated; signal transduction; transcription, DNA-dependent

Research Articles on STAT2

Similar Products

Product Notes

The STAT2 stat2 (Catalog #AAA2017772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Signal Transducer And Activator Of Transcription 2 (STAT2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Signal Transducer And Activator Of Transcription 2 (STAT2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the STAT2 stat2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPL RFLYPRIPRD EAFGCYYQEK VNLEEQEEYL KHKLIVISNR QVDELQQPLE LKQDSESLEV NAELLLAHDQ ELPLMMQTGL VLGTELKVDP ILSTAPQVLL EPAPQVLLEP APQVLLEPAP QVLLEPAPQV LLEPAPQVLL EPAPQVLLEP APQVQLEPAP QVLLELAPQV LLEPAPQVLL ELAPQVQLEP AHLLQQPSES DLPEDLQQIS VEDLKKLSNP STEYITTNEN PMLAGESSGD ETSIPYHSHF. It is sometimes possible for the material contained within the vial of "Signal Transducer And Activator Of Transcription 2 (STAT2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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