Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SHBG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) Polyclonal Antibody | anti-SIT1 antibody
Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1); Polyclonal Antibody; Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1); anti-SIT1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SIT1. It has been selected for its ability to recognize SIT1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRENNCTTA DLAWGIPSIT QAWGLWALFG VVTMLLLISL AALLSQWTRG RRRTQEEQGP PSGRSVEEVP LYGNLHYLQT GRLSEESRSE EQDPSSGGLA RGAEEATCYT SLQLRPAQGR IPSSGTPIKY CEVVLDSEPK
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRENNCTTA DLAWGIPSIT QAWGLWALFG VVTMLLLISL AALLSQWTRG RRRTQEEQGP PSGRSVEEVP LYGNLHYLQT GRLSEESRSE EQDPSSGGLA RGAEEATCYT SLQLRPAQGR IPSSGTPIKY CEVVLDSEPK
Sequence Length
196
Applicable Applications for anti-SIT1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SIT1 (Ser2~Pro175) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079379
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SHBG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SHBG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,368 Da
NCBI Official Full Name
signaling threshold-regulating transmembrane adapter 1
NCBI Official Synonym Full Names
signaling threshold regulating transmembrane adaptor 1
NCBI Official Symbol
Sit1
NCBI Protein Information
signaling threshold-regulating transmembrane adapter 1
UniProt Protein Name
Signaling threshold-regulating transmembrane adapter 1
Protein Family
UniProt Gene Name
Sit1
UniProt Synonym Gene Names
Sit
Uniprot Description
SIT: a low molecular weight transmembrane adaptor protein. A disulfide-linked homodimer. Expressed in lymphocytes. Following phosphorylation by Src and possibly Tec kinases, recruits the SH2 domain-containing tyrosine phosphatase SHP2 via an immunoreceptor tyrosine-based inhibition motif (ITIM).
Protein type: Adaptor/scaffold; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q22
Cellular Component: integral component of membrane; plasma membrane
Molecular Function: kinase binding; SH2 domain binding
Biological Process: adaptive immune response; regulation of T cell activation; signal transduction; T cell homeostasis
Similar Products
Product Notes
The SIT1 sit1 (Catalog #AAA2014348) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SIT1 sit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRENNCTTA DLAWGIPSIT QAWGLWALFG VVTMLLLISL AALLSQWTRG RRRTQEEQGP PSGRSVEEVP LYGNLHYLQT GRLSEESRSE EQDPSSGGLA RGAEEATCYT SLQLRPAQGR IPSSGTPIKY CEVVLDSEPK. It is sometimes possible for the material contained within the vial of "Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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