Heat Shock Transcription Factor 2 (HSF2) Recombinant Protein | HSF2 recombinant protein

Recombinant Heat Shock Transcription Factor 2 (HSF2)

Cat. Num. AAA2018540

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heat Shock Transcription Factor 2 (HSF2); Recombinant Heat Shock Transcription Factor 2 (HSF2); HSF2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP KYFKHNNMAS FVRQLNMYGF RKVVHIESGI VKQERDGPVE FQHPHFKQGQ DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQETIESRLSEL KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL
• Sequence Length: 513

• Applicable Applications for HSF2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Leu200 (Accession # Q9R120) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 13928966
• NCBI GeneID: 64441
• NCBI Accession #: NP_113882.1
• NCBI GenBank Nucleotide #: NM_031694.2
• UniProt Accession #: Q9R120; F1LQD4
• Molecular Weight: 27.3kDa
• NCBI Official Full Name: heat shock factor protein 2
• NCBI Official Synonym Full Names: heat shock transcription factor 2
• NCBI Official Symbol: Hsf2
• NCBI Protein Information: heat shock factor protein 2; heat shock factor 2
• UniProt Protein Name: Heat shock factor 2
• Protein Family: Heat shock factor protein
• UniProt Gene Name: Hsf2
• UniProt Synonym Gene Names: hsf2
• UniProt Entry Name: Q9R120_RAT

NCBI Description

DNA binding protein; involved in the transcriptional regulation of heat shock protein (hsp) genes [RGD, Feb 2006]

Uniprot Description

HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: cytoplasm; nucleus

Molecular Function: protein homodimerization activity; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis