Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse PAFAH1B3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) Polyclonal Antibody | anti-PAFAH1B3 antibody
Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3)
Gene Names
Pafah1b3; Pafahg; mus[g]
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3); Polyclonal Antibody; Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3); anti-PAFAH1B3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PAFAH1B3. It has been selected for its ability to recognize PAFAH1B3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSGEGENPAS KPTPVQDVQG DGRWMSLHHR FVADSKDKEP EVVFIGDSLV QLMHQCEIWR ELFSPLHALN FGIGGDSTQH VLWRLENGEL EHIRPKIVVV WVGTNNHSHT AEQVTGGIKA IVQLVNKLQP QARVVVLGLL PRGQHPNPLR EKNRQVNELV RAALAGYPRA HFLDADPGFV HSDGTISHHD MYDYLHLSRL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSGEGENPAS KPTPVQDVQG DGRWMSLHHR FVADSKDKEP EVVFIGDSLV QLMHQCEIWR ELFSPLHALN FGIGGDSTQH VLWRLENGEL EHIRPKIVVV WVGTNNHSHT AEQVTGGIKA IVQLVNKLQP QARVVVLGLL PRGQHPNPLR EKNRQVNELV RAALAGYPRA HFLDADPGFV HSDGTISHHD MYDYLHLSRL
Applicable Applications for anti-PAFAH1B3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PAFAH1B3 (Met1~Ser232) expressed in E.coli.
Cross Reactivity
Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054736
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse PAFAH1B3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse PAFAH1B3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,853 Da
NCBI Official Full Name
platelet-activating factor acetylhydrolase IB subunit gamma
NCBI Official Synonym Full Names
platelet-activating factor acetylhydrolase, isoform 1b, subunit 3
NCBI Official Symbol
Pafah1b3
NCBI Official Synonym Symbols
Pafahg; mus[g]
NCBI Protein Information
platelet-activating factor acetylhydrolase IB subunit gamma
UniProt Protein Name
Platelet-activating factor acetylhydrolase IB subunit gamma
Protein Family
UniProt Gene Name
Pafah1b3
UniProt Synonym Gene Names
Pafahg; PAF-AH 29 kDa subunit; PAFAH subunit gamma
Uniprot Description
PAFAH1B3: Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
Protein type: EC 3.1.1.47; Lipase; Lipid Metabolism - ether lipid
Chromosomal Location of Human Ortholog: 7|7 A3
Cellular Component: cytoplasm; cytosol; membrane
Molecular Function: 1-alkyl-2-acetylglycerophosphocholine esterase activity; hydrolase activity; identical protein binding; platelet-activating factor acetyltransferase activity; protein binding; protein heterodimerization activity
Biological Process: brain development; lipid catabolic process; lipid metabolic process; spermatogenesis
Research Articles on PAFAH1B3
Similar Products
Product Notes
The PAFAH1B3 pafah1b3 (Catalog #AAA2014926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PAFAH1B3 pafah1b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSGEGENPAS KPTPVQDVQG DGRWMSLHHR FVADSKDKEP EVVFIGDSLV QLMHQCEIWR ELFSPLHALN FGIGGDSTQH VLWRLENGEL EHIRPKIVVV WVGTNNHSHT AEQVTGGIKA IVQLVNKLQP QARVVVLGLL PRGQHPNPLR EKNRQVNELV RAALAGYPRA HFLDADPGFV HSDGTISHHD MYDYLHLSRL. It is sometimes possible for the material contained within the vial of "Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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