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Western Blot (WB) (Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse GAL Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Galanin (GAL) Polyclonal Antibody | anti-GAL antibody

Polyclonal Antibody to Galanin (GAL)

Gene Names
Gal; Galn
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Galanin (GAL); Polyclonal Antibody; Polyclonal Antibody to Galanin (GAL); anti-GAL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GAL. It has been selected for its ability to recognize GAL in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LNSAG YLLGPHAIDN HRSFSDKHGL TGKRELQLEV EERRPGSVDV PLPESNIVRT IMEFLSFLHL KEAGALDSLP
Applicable Applications for anti-GAL antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GAL (Leu36~Lys123) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051427
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse GAL Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse GAL Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,471 Da
NCBI Official Full Name
galanin peptides isoform 1 preproprotein
NCBI Official Synonym Full Names
galanin
NCBI Official Symbol
Gal
NCBI Official Synonym Symbols
Galn
NCBI Protein Information
galanin peptides
UniProt Protein Name
Galanin peptides
Protein Family
UniProt Gene Name
Gal
UniProt Synonym Gene Names
Galn; GMAP

NCBI Description

This gene encodes a neuroendocrine peptide that is principally produced by a subpopulation of lactotrophs in the pituitary gland. The encoded protein is a precursor that is proteolytically processed to generate two mature peptides: galanin and galanin message-associated peptide (GMAP). Mice lacking the encoded protein fail to lactate sufficiently due to abnormalities in the expression of prolactin and lactotroph proliferation, exhibit attenuated chronic neuropathic pain and developmental deficits in the dorsal root ganglion neurons. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature proteins. [provided by RefSeq, Jul 2016]

Uniprot Description

Galanin: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion. Belongs to the galanin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19 A|19 3.16 cM

Cellular Component: cell soma; cytoplasm; extracellular region; extracellular space; Golgi apparatus; secretory granule

Molecular Function: galanin receptor activity; galanin receptor binding; hormone activity; neuropeptide hormone activity; type 1 galanin receptor binding; type 2 galanin receptor binding; type 3 galanin receptor binding

Biological Process: cAMP-mediated signaling; feeding behavior; inflammatory response; negative regulation of cell proliferation; negative regulation of lymphocyte proliferation; nervous system development; neuropeptide signaling pathway; positive regulation of apoptosis; positive regulation of catagen; positive regulation of cortisol secretion; positive regulation of large conductance calcium-activated potassium channel activity; positive regulation of transcription from RNA polymerase II promoter; protein kinase A signaling; regulation of glucocorticoid metabolic process

Research Articles on GAL

Similar Products

Product Notes

The GAL gal (Catalog #AAA2015223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Galanin (GAL) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Galanin (GAL) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GAL gal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LNSAG YLLGPHAIDN HRSFSDKHGL TGKRELQLEV EERRPGSVDV PLPESNIVRT IMEFLSFLHL KEAGALDSLP. It is sometimes possible for the material contained within the vial of "Galanin (GAL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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