Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse LRRC32 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Leucine Rich Repeat Containing Protein 32 (LRRC32) Polyclonal Antibody | anti-LRRC32 antibody
Polyclonal Antibody to Leucine Rich Repeat Containing Protein 32 (LRRC32)
Gene Names
Lrrc32; Garp; AI426318; EG434215; D11S833Eh; D7H11S833E
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Leucine Rich Repeat Containing Protein 32 (LRRC32); Polyclonal Antibody; Polyclonal Antibody to Leucine Rich Repeat Containing Protein 32 (LRRC32); anti-LRRC32 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LRRC32. It has been selected for its ability to recognize LRRC32 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Q ELPPYTFASL ASLQRLNLQG NQVSPCGGPA EPGPPGCVDF SGIPTLHVLN MAGNSMGMLR AGSFLHTPLT ELDLSTNPGL DVATGALVGL EASLEVLELQ GNGLTVLRVD LPCFLRLKRL NLAENQLSHL PAWTRAVSLE VLDLRNNSFS LLPGNAMGGL ETSLRRLYLQ GNPLSCCGNG WLAAQLHQGR VDVDATQDLI
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Q ELPPYTFASL ASLQRLNLQG NQVSPCGGPA EPGPPGCVDF SGIPTLHVLN MAGNSMGMLR AGSFLHTPLT ELDLSTNPGL DVATGALVGL EASLEVLELQ GNGLTVLRVD LPCFLRLKRL NLAENQLSHL PAWTRAVSLE VLDLRNNSFS LLPGNAMGGL ETSLRRLYLQ GNPLSCCGNG WLAAQLHQGR VDVDATQDLI
Sequence Length
662
Applicable Applications for anti-LRRC32 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant LRRC32 (Gln400~Leu643) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2084735
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse LRRC32 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse LRRC32 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,418 Da
NCBI Official Full Name
leucine-rich repeat-containing protein 32
NCBI Official Synonym Full Names
leucine rich repeat containing 32
NCBI Official Symbol
Lrrc32
NCBI Official Synonym Symbols
Garp; AI426318; EG434215; D11S833Eh; D7H11S833E
NCBI Protein Information
leucine-rich repeat-containing protein 32
UniProt Protein Name
Leucine-rich repeat-containing 32
Protein Family
UniProt Gene Name
Lrrc32
Uniprot Description
LRRC32:
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 7 E1|7 53.86 cM
Cellular Component: integral component of plasma membrane
Biological Process: negative regulation of activated T cell proliferation; negative regulation of cytokine secretion
Research Articles on LRRC32
Similar Products
Product Notes
The LRRC32 lrrc32 (Catalog #AAA2014820) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Leucine Rich Repeat Containing Protein 32 (LRRC32) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Leucine Rich Repeat Containing Protein 32 (LRRC32) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LRRC32 lrrc32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Q ELPPYTFASL ASLQRLNLQG NQVSPCGGPA EPGPPGCVDF SGIPTLHVLN MAGNSMGMLR AGSFLHTPLT ELDLSTNPGL DVATGALVGL EASLEVLELQ GNGLTVLRVD LPCFLRLKRL NLAENQLSHL PAWTRAVSLE VLDLRNNSFS LLPGNAMGGL ETSLRRLYLQ GNPLSCCGNG WLAAQLHQGR VDVDATQDLI. It is sometimes possible for the material contained within the vial of "Leucine Rich Repeat Containing Protein 32 (LRRC32), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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