Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CASP12 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Caspase 12 (CASP12) Polyclonal Antibody | anti-CASP12 antibody
Polyclonal Antibody to Caspase 12 (CASP12)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MAAKRTHERD PIYKIKGLAK DMLDGVFDDL MDKNVLNGDE LLKIGEGASL ILSKAENLVE SFFEKTEMAG KIFAGHIANS DKQLSLQFPS DDEEDELQKM FTPSSASESR GKVEDEEMEV NVGVAHASHL MLTVPQGIQS TEVQDSLKLC SRDWFCTMKT ERAEEIYPVM EKEGRTRLAL IICNKKFDYL FDRDDAETDI
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CASP12 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat CASP12 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI Description
cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis [RGD, Feb 2006]
Uniprot Description
CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.22.-; Protease
Chromosomal Location of Human Ortholog: 8q11
Cellular Component: cytosol; endoplasmic reticulum; intracellular membrane-bound organelle; nucleus
Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; protease binding; protein binding
Biological Process: aging; apoptosis; endoplasmic reticulum unfolded protein response; positive regulation of apoptosis; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; protein autoprocessing; proteolysis; regulation of apoptosis; regulation of inflammatory response; response to anoxia; response to cold; response to drug; response to ethanol; response to inorganic substance; response to iron(III) ion; response to nicotine; response to organonitrogen compound
Research Articles on CASP12
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Product Notes
The CASP12 casp12 (Catalog #AAA2015420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Caspase 12 (CASP12) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 12 (CASP12) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CASP12 casp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MAAKRTHERD PIYKIKGLAK DMLDGVFDDL MDKNVLNGDE LLKIGEGASL ILSKAENLVE SFFEKTEMAG KIFAGHIANS DKQLSLQFPS DDEEDELQKM FTPSSASESR GKVEDEEMEV NVGVAHASHL MLTVPQGIQS TEVQDSLKLC SRDWFCTMKT ERAEEIYPVM EKEGRTRLAL IICNKKFDYL FDRDDAETDI. It is sometimes possible for the material contained within the vial of "Caspase 12 (CASP12), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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